中国烟草学报2018，Vol.24Issue(1)：38-44,7.DOI:10.16472/j.chinatobacco.2017.170
低氮胁迫对烟草生物学性状、生长素及NtPINs基因家族的影响
Effects of nitrogen deficiency stress on tobacco biological characters,auxin and NtPINs gene family
摘要
Abstract
This paper investigated the regulatory effect of auxin on growth of tobacco root and glandular trichome under nitrogen deficiency stress. Sand culture experiment was designed to study effects of nitrogen deficiency stress on tobacco biomass, nitrogen concentration, leaf and root development, auxin concentration, expression of the family gene NtPINs, and efflux protein of auxin. Results showed that, compared with normal nitrogen supply level (NH4NO3:2.5 mmol/L), the biomass of upper leaves, stems and roots decreased significantly under nitrogen deficiency stress (NH4NO3:0.25 mmol/L). Total nitrogen concentration in shoots decreased by about 30%, and that of roots by about 40.2%. The density of trichome of upper leaves increased by 13.9%, and that of middle and lower leaves decreased by 40% and 31.9% respectively. The content of auxin in upper leaves increased by 26%, while that of middle leaves and root system decreased by 7.1% and 13.1% respectively, and that of lower leaves showed no obvious differences. Results of qRT-PCR showed that the expression of NtPIN1,NtPIN3,NtPIN4 and NtPIN9 genes in tobacco roots under nitrogen deficiency stress was significantly lower than that in the control group (P<0.05). It was concluded that under nitrogen deficiency tress, the growth of auxin in tobacco leaves from upper part of the ground (upper leaves, middle leaves and lower leaves) to the root system is the key factor for the occurrence of glandular trichome and change of root architecture.关键词
烟草根系/腺毛/生长素/低氮胁迫Key words
tobacco root/glandular trichome/auxin/nitrogen deficiency stress引用本文复制引用
王立志,魏跃伟,黄明月,朱彦铭,丁永乐,贾宏昉..低氮胁迫对烟草生物学性状、生长素及NtPINs基因家族的影响[J].中国烟草学报,2018,24(1):38-44,7.基金项目
河南省高等学校重点科研项目(15A210029) （15A210029）
国家自然科学基金(31303817) （31303817）
贵州省烟草公司毕节市公司科技攻关项目(毕节合2014-59) （毕节合2014-59）
