中国烟草学报2018，Vol.24Issue(1)：60-67,8.DOI:10.16472/j.chinatobacco.2017.263
打顶对烟草叶片多酚代谢及其关键酶的影响
Effect of topping on polyphenols metabolism and key enzymes in tobacco leaves
摘要
Abstract
Effect of topping on polyphenols metabolism, content of polyphenols and relevant key enzyme activities and gene expression using topping treatments on different periods (seedling stage and topping stage) in Honghuadajinyuan were tested by high performance liquid chromatography (HPLC), ultraviolet absorption spectrophotometry and quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Compared with un-topped tobacco plants, topping at the 30th day after transplanting could lead to significant decrease of polyphenols such as chlorogenic acid in leaves, and the activity of PAL, PPO and POD increased, and 4CL activity decreased, while the activity of C4H was not significantly different from control.The expression of PAL1,PAL4 and 4CL2 were up-regulated.Topping at the 85th day after transplanting could significantly improve content of polyphenols in leaves, PAL, C4H, 4CL and POD activity increased, the activity of PPO decreased.The expression of PAL1 was down-regulated,and the expression of 4CL2 was not significantly different from control.Expression levels of PAL2,PAL3,C4H and 4CL1 were up-regulated in two periods.The above results showed that polyphenols biosynthesis in tobacco leaves induced by topping was related to growth period. Polyphenols content after topping was negatively correlated with activity of PPO, and positively correlated with activity of 4CL. At seedling stage the degradation rate of polyphenols in tobacco leaves was stronger than that in synthesis rate, resulting in the decrease of polyphenol content after topping. On the contrary, the synthesis rate of polyphenols in tobacco leaves was stronger than that in degradation rate after flower-bud appeared, which resulted in the increase of polyphenols content after topping.关键词
烟草/打顶/生育期/多酚/基因表达Key words
tobacco/topping/growth stage/polyphenols/gene expression引用本文复制引用
杨银菊,张彦,王树声,刘光亮,许娜,王程栋,周培禄,陈爱国..打顶对烟草叶片多酚代谢及其关键酶的影响[J].中国烟草学报,2018,24(1):60-67,8.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC03) （ASTIP-TRIC03）
龙岩市烟草公司项目"龙岩特色烟叶开发"(201302) （201302）
2017年度中国农业科学院烟草研究所青年科学基金项目"生长素参与调控干旱胁迫下烟草根系生长的机制"(2017B01) （2017B01）
