中国医院用药评价与分析2018，Vol.18Issue(2)：208-210,3.DOI:10.14009/j.issn.1672-2124.2018.02.021
顶空气相色谱法测定生脉注射液中乙醇残留量
Determination of Alcohol Residue in Shengmai Injection by Headspace Gas Chromatography
龚源 1吴杰1
作者信息
- 1. 复旦大学附属金山医院药剂科,上海 201508
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for determination of alcohol residue in shengmai injection by headspace gas chromatography .METHODS:The alcohol residue in shengmai injection was separated by HP-5 column (30 m ×0.32 mm,0.25 μm);the carrier gas was nitrogen , the flow rate was 1 ml/min.The splitting ratio was 1:1. The temperature of detector and injector was 250 ℃ and 200 ℃, respectively .The temperature of column was maintained at 50 ℃ for 6 min.The containers of headspace injector were in equilibrium at 80 ℃ for 10 min.The injector volume was 1 ml.RESULTS: Under chromatographic condition , the theoretical plate number of ethanol ≥160000, and the separation degree of ethanol peak and adjacent peak ≥3.0.The alcohol concentration was good in the range of 0.001％-0.020％, with limit of detection of 0.00006％( V/V ) .CONCLUSIONS: The established method is simple , sensitive and accurate with good reproducibility , which can be used to determine the alcohol residue in shengmai injection .关键词
顶空气相色谱法/生脉注射液/乙醇残留量Key words
Headspace gas chromatography/Shengmai injection/Alcohol residue分类
医药卫生引用本文复制引用
龚源,吴杰..顶空气相色谱法测定生脉注射液中乙醇残留量[J].中国医院用药评价与分析,2018,18(2):208-210,3.
