植物学报2018，Vol.53Issue(1)：42-50,9.DOI:10.11983/CBB17058
拟南芥组蛋白分子伴侣AtHIRA参与体细胞同源重组及盐胁迫响应
Histone Chaperone AtHIRA is Involved in Somatic Homologous Recombination and Salinity Response in Arabidopsis
摘要
Abstract
Histone regulator (HIRA) is a specific chaperone for histone H3.3 and plays an important role in the incorporation of histone H3.3 into chromatin.HIRA is indispensable in mammalian embryo development and DNA damage repair process,but we have few studies of the function of an HIRA homolog in plants.Here,we studied the function of Arabi dopsis thaliana AtHIRA in somatic homologous recombination (HR) and meiotic homologous recombination.We used the somatic HR system and the meiotic homologous recombination system in wild type and the hira-1 mutant,a lossof-function mutant of AtHIRA.Both intramolecular and intermolecular HR frequency was lower in hira-1 than the wild type under normal growth conditions and under bleomycin or UV-C treatment,with no significant difference in the frequency of meiotic recombination of microsporocytes between the wild type and hira-1 mutant under normal growth conditions.As well,under normal growth conditions or bleomycin treatment,loss-of-function of AtHIRA in Arabidopsis did not affect the DNA damage level.On qRT-PCR,the expression of RAD51 and RAD54,two DNA repair-related genes,was higher in hira-1 than the wild type.In addition,hira-1 had a salt-sensitive phenotype as compared with the wild type under salt stress.AtHIRA may play a role in somatic HR and the salinity response in Arabidopsis.关键词
HIRA/同源重组/DNA损伤修复/减数分裂/盐胁迫Key words
HIRA/homologous recombination/DNA damage repair/meiotic/salt stress引用本文复制引用
陈成,董爱武,苏伟..拟南芥组蛋白分子伴侣AtHIRA参与体细胞同源重组及盐胁迫响应[J].植物学报,2018,53(1):42-50,9.基金项目
国家自然科学基金(No.31671263) （No.31671263）
