植物学报2018，Vol.53Issue(1)：94-103,10.DOI:10.11983/CBB16247
鲁桑叶绿体基因组序列及特征分析
Complete Chloroplast Genome Sequence and Characteristics Analysis of Morus multicaulis
摘要
Abstract
Mulberry is an economically important crop in Asia.We determined the complete chloroplast sequence of cultivated species of Morus multicaulis.Ribenhuchen was used as experimental material.High-throughput sequencing was used to sequence the chloroplast genome and the genome structure (NCBI No.:KU355297),and we compared the chloroplast genome with those of reported sibling species (Morus mongolica,M.indica,M.notabilis).The chloroplast genome (cpDNA) of M.multicaulis with a typical quadripartite structure is 159 154 bp long.The cpDNA of M.multicaulis contains 130 genes,including 85 protein coding genes (18 genes duplicated in the inverted repeat regions),37 transfer RNA genes and 8 ribosomal RNA genes.There are 82 simple sequence repeats,and the number of mono-,di-,tri-,tetra-,pentanucleotide repeat motifs is 63,7,2,9,and 1,with no hexanucleotide repeat sequences.Mono-nucleotide repeat sequences accounted for 76.8％ of the cpDNA of simple sequence repeats.MEGA 6.0 was used to construct the phyIogenetic tree of 15 species and for cluster analysis of Morus plants.M.multicaulis and M.mongolica were clustered into one group.The research results have reference value for chloroplast genome research,molecular marker development and breeding of mulberry.关键词
鲁桑/叶绿体基因组/高通量测序/聚类分析Key words
Morus multicaulis/chloroplast genome/high-throughput sequencing/cluster analysis引用本文复制引用
李巧丽,延娜,宋琼,郭军战..鲁桑叶绿体基因组序列及特征分析[J].植物学报,2018,53(1):94-103,10.基金项目
西北农林科技大学唐仲英育种基金(No.2013-14) （No.2013-14）
