安徽农业科学2017，Vol.45Issue(34)：78-80,122,4.
具有潜在抑制肠道致病菌能力的乳酸菌的筛选
Screening of Lactic Acid Bacteria with the Ability of Inhibiting Enteropathogenic Bacteria in Potential
摘要
Abstract
[Objective] Lactic acid bacteria (LAB) with strong resistance of acid and bile salt were screened from laboratory,and their adhesion ability of intestinal epithelial cells and inhibition ability to intestinal pathogens were studied.[Method]The resistance of acid and bile salt and the adhesion ability of IEC-6 cells were studied by artificial gastrointestinal fluid and co-cultured with IEC-6 cells,respectively,and the bacteriostatic effect was deter mined by single agar plate diffusion method.[Result]The survival rate of 10 strains were cultured 3 h in pH 3.0 artificial gastric juice was greater than 44.00％,and cultured 4h in pH 8.0 artificial intestinal juice was greater than 51.56％,and cultured 24 h in medium containing 0.30％ bile salts was greater than 20.00％;the adhesion number of C13,A03,A17 and A90 on IEC-6 cells was greater than 8 cells;the inhibiting capacity of C13,A03 on Clostridium difficile,Salmonella and Escherichia coli was stronger than others,the antibacterial circle diameter was greater than 22 mm.C13 and A03 were identified as Lactobacillus rhanmosu and Lactobacillus plantarum by 16S rRNA sequencing,respectively.[Conclusion] L.rhamnosu C13,L.plantarum A03 with the strong ability of acid and bile salt resistance and adhesion to epithelial cells,which have good inhibitory effect on pathogenic bacteria,and can be used as a fit probiotic candidate strain.关键词
乳酸菌/耐酸耐胆盐/黏附/抑菌Key words
Lactic acid bacteria/Resistance of acid and bile salt/Adhesion/Inhibition of pathogenic bacteria分类
轻工纺织引用本文复制引用
陈大卫,刘莎莎,黄玉军,郭蕾,张蓉..具有潜在抑制肠道致病菌能力的乳酸菌的筛选[J].安徽农业科学,2017,45(34):78-80,122,4.基金项目
国家自然科学基金青年基金项目(31701627) （31701627）
江苏省高校自然科学研究面上项目(17KJB550009) （17KJB550009）
江苏省高校自然科学研究重大项目(17KJA550004) （17KJA550004）
扬州大学2017年大学生科技创新基金项目(乳酸菌的有机千禧果保鲜及其作用机制研究). （乳酸菌的有机千禧果保鲜及其作用机制研究）
