安徽农业科学2017，Vol.45Issue(34)：144-147,4.
一种基于TaqMan探针法的阪崎肠杆菌qPCR全自动检测技术体系的建立
Establishment of qPCR Automatic Detection System Based on TaqMan Probe Method for Enterobacter sakazakii
摘要
Abstract
[Objective]To establish a rapid and accurate detection system for foodbome pathogens in food samples.[Method] A qPCR detection system for Enterobacter sakazakii based on TaqMan probe method was established,which was supported by the microbial molecular detector researched by key laboratory of Huzhou mocrobilogical agents and agricultural products safety.[Result]The system was successfully established and the standard curve slope,R2,amplification efficiency was-3.44,0.995 5,95.3％,respectively.[Conclusion] The qPCR detection system has strong specificity,high sensitivity,good stability and repeatability,which could shorten the detection time greatly.The system provides a new way for high throughput and automated detection of E.sakazakii in food.关键词
阪崎肠杆菌/qPCR/全自动检测Key words
Enterobacter sakazakii/qPCR/Automatic detection分类
轻工纺织引用本文复制引用
王芬,刘宽,马龙,李阳,吴酬飞..一种基于TaqMan探针法的阪崎肠杆菌qPCR全自动检测技术体系的建立[J].安徽农业科学,2017,45(34):144-147,4.基金项目
浙江省食药监管系统科技计划项目(SP201618) （SP201618）
浙江省大学生科技创新活动计划(新苗人才计划)项目(2016R427009). （新苗人才计划）
