安徽农业科学2017，Vol.45Issue(34)：155-159,5.
2种果蝇取食后樱桃可培养微生物多样性的动态变化
Dynamics of Microbial Diversity in Cherry Fed by Two Species of Drosophila
摘要
Abstract
[Objective] The aim was to understand the dynamics of microbial diversity in natural cherry and the cherry treated by Drosophila suzukii and Drosophila melanogaster.[Method] The dynamics of microbial diversity in natural cherry and the cherry treated by Drosophila suzukii and Drosophila melanogaster,separately,and together,microorganisms in cherry treated by D.suzukii and D.melanogaster,for 2,4,6,8,10,12 days were separated,purified,and identified through ITS and 16S rDNA genetic analysis.[Result] The Results showed that,16 bacterial species and 12 fungi species were identified from all the materials.The dynamic of microbial diversity was changed with the increasing of time.The microorganism species was abundant from 8 to 12 day under the natural condition.The dominant populations of bacteria were Pseudomonas fluorescem and Staphylococcus sp..After fed by D.melanogaster for 6 days,the largest number of microorganism species occurred at the 6th day and the bacteria of Firmicutes were dominant populations.While fed by D.suzukii or by two species of Drosophila together,the microorganism species increased from 2 to 6 day.It was concluded that,the decay of cherry was accelerated due to that the microbial diversity in cherry was changed by D.suzukii.D.melanogaster formed continuing harm through the mechanical injury from D.suzukii.in which the dominant populations of bacteria were the bacteria of Proteobacteria.Moreover,Geotrichum candidum was the steady and dominant fungus in cherry treated by Drosophila species but absent in the natural cherry.[Conclusion] This study provides a theoretical foundation for investigating coevolution among cherry,Drosophila sp.and microorganisms.关键词
斑翅果蝇/黑腹果蝇/樱桃/微生物多样性/动态Key words
Drosophila suzukii/Drosophila melanogaster/Cherry/Microbial diversity/Dynamics分类
生物科学引用本文复制引用
高欢欢,王文博,吕召云,刘倩,翟一凡,李丽莉,郑礼,于毅..2种果蝇取食后樱桃可培养微生物多样性的动态变化[J].安徽农业科学,2017,45(34):155-159,5.基金项目
山东省农业科学院农业科技创新工程(CXGC2016B11,CXGC2016A09) （CXGC2016B11,CXGC2016A09）
山东省自主创新及成果转化专项(2014ZZCX02703) （2014ZZCX02703）
山东省农业科学院重大科技成果培育计划“果树食心虫灾变机制与减量化防控技术研究与应用” （）
国家农产品质量安全风险评估重大专项(GJFP201601302). （GJFP201601302）
