安徽农业科学2018，Vol.46Issue(8)：15-18,45,5.
卷烟加工及储存过程中细菌群落组成及变化研究
Effects of Cigarette Processing and Storage Process on Bacterial Community Structure
摘要
Abstract
[Objective]In order to study the changes of bacterial community structure and diversity in cigarette processing and storage process [Method]The two generation high-throughput sequencing platforms were used to study the processing technology of Yunyan tobacco(R)and the surface microorganism of tobacco leaves during storage.The 16S rRNA gene was sequenced by high-throughput sequencing.[Result]Although dif-ferent technics in the same processing in the process of microbial community richness was not the same,but the main types of high richness micro-bial communities were consistent.In addition,the results also showed that the storage time of tobacco leaves could be the main factor affecting the changing of microbial community rather than the cities where they storage.[Conclusion]The study has a certain reference significance for impro-ving tobacco processing technology from microorganism angle.Meanwhile,it indicates that the storage time of tobacco leaves may be the main fac-tor that affects the later stage of microbial community.关键词
制丝工艺/高通量测序/微生物群落结构/烟叶品质Key words
Cigarette processing/High-throughput sequencing/Microbial community structure/Tobacco quality分类
轻工纺织引用本文复制引用
陈兴,杨莹,李珊辉,张天栋,颜克亮,刘晓敏,肖敏,陈微..卷烟加工及储存过程中细菌群落组成及变化研究[J].安徽农业科学,2018,46(8):15-18,45,5.基金项目
中国烟草总公司科技重点项目(110201402042) （110201402042）
云南中烟工业有限责任公司科技项目(2015CP01) （2015CP01）
