安徽农业科学2018，Vol.46Issue(8)：108-109,117,3.
一株产脂肪酶芽胞杆菌菌株SJXYZ的鉴定
Identification of a Lipase-producing Bacillus sp.SJXYZ
摘要
Abstract
[Objective]To identify strain SJXYZ,which was isolated from commercial sterilized milk.[Method]Strain SJXYZ was character-ized by morphological, biochemical and physiological tests,16S rRNA gene sequence determination and phylogenetic analyses.[Result]16S rRNA gene analysis indicated that strain SJXYZ belonged to the genus Bacillus and had small higher than 97% similarity to B.altitudinis 41KF2bT, B.xiamenensis HYC-10T, B.safensis FO-36bT, B.zhangzhouensis DW5-4T, B.australimaris NH7I_1T and B.pumilus ATCC 7061T.The phylogenetic analysis showed that strain SJXYZ was closest to B.altitudinis 41KF2bT,but formed an independent branch separa-ted from all the reference strains.Cells were gram-stain-positive,straight rod,motile.Growth occurred at pH 5-9,at 0%-5% NaCl,and with optimum temperature at 30 -35 ℃.Lipase was passive.Proteinase, cellulase and amylase were negative.Sensitive to ampicillin (10 μg), gentamycin(10 μg), norxacin(10 μg), cefazolin(30 μg), tetracycline(30 μg), cephalothin(30 μg)and streptomycin (10 μg).Genomic and phenotypic data of strain SJXYZ shew a big difference from B.altitudinis 41KF2bT.[Conclusion]On the basis of these findings, we propose that strain SJXYZ represents a new species of Bacillus for which we propose the name Bacillus sp.SJXYZ.关键词
芽胞杆菌/菌种鉴定/脂肪酶Key words
Bacillus/Identification/Lipase分类
农业科技引用本文复制引用
陈亮,陈明霞,李和阳..一株产脂肪酶芽胞杆菌菌株SJXYZ的鉴定[J].安徽农业科学,2018,46(8):108-109,117,3.基金项目
国家自然科学基金项目(41506179) （41506179）
福建省自然科学基金项目(2015J01613) （2015J01613）
"全球变化与海气相互作用"专项(GASI-03-01-03 -01,GASI-03 -01 -03 -02) （GASI-03-01-03 -01,GASI-03 -01 -03 -02）
华侨大学高层次人才科研启动项目(12BS206) （12BS206）
