安徽中医药大学学报2017，Vol.36Issue(6)：44-49,6.DOI:10.3969/j.issn.2095-7246.2017.06.014
通督调神针灸法对局灶性脑缺血再灌注模型大鼠皮质区CD34、CD133及血清VEGFR-2表达的影响
Effect of Tongdu Tiaoshen Acupuncture on the Cortical Expression of CD34 and CD133 and Serum Expression of VEGFR-2 in A Rat Model of Middle Cerebral Artery Occlusion/Reperfusion
摘要
Abstract
Objectivc To investigate the effect of Tongdu Tiaoshen acupuncture on brain protection and angiogenesis in a rat model of middle cerebral artery occlusion/reperfusion (MCAO/R).Methods One hundred and fifty Sprague-Dawley rats were equally and randomly divided into sham operation group,Tongdu Tiaoshen acupuncture group,conventional acupuncture group,drug treatment group,and model control group.The Tongdu Tiaoshen acupuncture group received moxibustion treatment at Baihui (DU20) and bloodletting puncture at Dazhui (DU14).The conventional acupuncture group received punctures at Fengchi (GB20),Quchi (LI11),Hegu (LI4),Tsusanli (ST36),and Sanyinjiao (SP6).The drug treatment group received Tongxinluo Capsule by gavage at a daily dose of 1.0 g/kg.The sham operation group and the model control group received no treatment.The other groups received treatment once a day for a total of 28 days.The rats were sacrificed at 7,14,21,and 28 days and the brain tissues were collected.The expression of CD34 and CD133 in the ischemic cortex was detected on paraffin-embedded brain tissue sections by immunohistochemistry.The expression of VEGFR-2 was determined by enzyme-linked immunosorbent assay.Results In the MCAO/R rats,the expression of CD34 and CD133 in the ischemic cortex and serum expression of VEGFR-2 were the highest at 7 days and gradually decreased afterwards.Tongdu Tiaoshen acupuncture,conventional acupuncture,and Tongxinluo Capsule significantly elevated the expression of CD34 and CD133 in the ischemic cortex and serum expression of VEGFR-2 in the MCAO/R rats (all P＜0.05).Moreover,Tongdu Tiaoshen acupuncture had significantly better efficacy than conventional acupuncture and Tongxinluo Capsule (all P＜0.05).Conclusion Tongdu Tiaoshen acupuncture is an effective approach for promoting the expression of CD34 and CD133 in the ischemic cortex and serum expression of VEGFR-2 in the MCAO/R rats.关键词
大脑中动脉缺血再灌注模型/通督调神针灸法/CD34/CD133/VEGFR-2Key words
Middle cerebral artery occlusion/reperfusion model/Tongdu Tiaoshen acupuncture/CD34/CD133/VEGFR-2分类
医药卫生引用本文复制引用
王保国,马月,孔红兵,贾泽坤,曹奕..通督调神针灸法对局灶性脑缺血再灌注模型大鼠皮质区CD34、CD133及血清VEGFR-2表达的影响[J].安徽中医药大学学报,2017,36(6):44-49,6.基金项目
安徽中医药大学青年基金项目(2014qn035) （2014qn035）
