吉林大学学报（医学版）2018，Vol.44Issue(1)：8-12,5.DOI:10.13481/j.1671-587x.20180102
慢性束缚应激对老龄大鼠空间学习和记忆能力及海马齿状回区兴奋性氨基酸水平的影响
Effects of chronic restraint stress on abilities of spatial learning and memory and levels of excitatory amino acids in hippocampal dentate gyrus in old rats
摘要
Abstract
Objective:To explore the effects of chronic restraint stress (CRS) on the abilities of spatial learning and memory and the levels of excitatory amino acids in the hippocampal dentate gyrus (DG) in the old rats,and to investigate the neurochemical mechanism of CRS in affecting the spatial learning and memory abilities.Methods:Sixteen male SD rats (18 months old) were randomly divided into control group (n =8) and CRS group (n=8),and the rats in CRS group received CRS 2 h every day for 30 d.And then the spatial learning and memory abilities of rats were measured by Morris water maze (MWM) test,and the extracellular levels of excitatory amino acids including asparate (Asp) and glutamate (Glu) in the DG were simultaneously determined by in vivo microdialysis and HPLC.The levels of corticosterone (CORT) and epinephrine (EPI) in serum of the rats wereexamined by ELISA assay.Results:In CRS group,the escape latencies on the 2nd-4th days were significantly increased and the percentage of time spent in target quadrant on the 5th day was markedly decreased in MWM test compared with control group (P＜0.05).Compared with before training,the extracelluar level of Asp in the DG in control group was significantly increased on the 2nd day in MWM test;compared with control group,the extracelluar level of Asp in the DG in CRS group was significantly decreased on the 3rd day in MWM test (P＜0.05).Compared with before training,the Glu levels in the DG in MWM test in both control and CRS groups were markedly increased (P＜0.05),but there was no significant difference between two groups (P＞0.05).Compared with control group,the levels of CORT and EPI in the serum of the rats in CRS group were significantly increased (P＜0.05).Conclusion:CRS impairs the spatial learning and memory abilities in the old rats,which may be related to the decrease of Asp level in the hippicampal DG of the rats.关键词
慢性束缚应激/海马齿状回/空间学习/记忆能力/兴奋性氨基酸/老龄大鼠Key words
chronic restraint stress/hippocampal dentate gyrus/spatial learning and memory abilities/excitatory amino acids/old rats分类
生物科学引用本文复制引用
王玮瑶,陈玲,岳学玲,金清华..慢性束缚应激对老龄大鼠空间学习和记忆能力及海马齿状回区兴奋性氨基酸水平的影响[J].吉林大学学报（医学版）,2018,44(1):8-12,5.基金项目
国家自然科学基金资助课题(31560278) （31560278）
吉林省教育厅“十三五”科学技术研究项目资助课题(2016-262) （2016-262）
