吉林大学学报（医学版）2018，Vol.44Issue(1)：13-17,后插3,6.DOI:10.13481/j.1671-587x.20180103
十全大补汤对SV40T抗原转基因小鼠晶状体自发肿瘤的抑制作用及其分子机制
Inhibitory effect of Shiquandabu decoction on spontaneous len tumor of SV40 T antigen transgenic mice and its molecular mechanisms
摘要
Abstract
Objective:To explore the inhibitory effect of Shiquandabu decoction on the spontaneous len tumor of the SV40 T antigen transgenic (TG) mice,and to clarify its molecular mechanism.Methods:The SV40 T antigen TG mice were randomly divided into control group (n=39) and drug treatment group (n=25).The mice in control group were fed normally,while the mice in drug treatment group were fed with Shiquandabu decoction at the 3rd week after birth,the survival time of mice was recorded.Three mice in control group and drug treatment group were randomly chosen to collect the blood from the tail vein and the amino acid levels were measured respectively 8 and 15 weeks after Shiquandabu decoction administration.Then the mice were sacrificed and the liver tissue wascollected.Gene chip hybridization was used to detect the differences in the expressions of ribosomal function related genes in liver tissue of the mice in two groups and the related signal pathway was explored.Results:The survival analysis demonstrated that the survival rate of TG mice in drug treatment was higher than that in control group (P＜0.05).Compared with control group,the serum levels of alanine,valine,leucine,isoleucine,threonine,methionine,proline,tyrosine,lysine,sarcosine,citrulline,ornithine and hydroxylysine of the mice in drug treatment group 8 weeks after administration of Shiquandabu decoction were increased (P＜0.05);and the serum levels of cystathionine,taurine,methylhistidine,anserine and ethanolamine were decreased (P＜0.05).Fifteen weeks after administration,compare with control group,the serum levels of threonine and citrulline of the mice in drug teeatment group were increased (P＜0.05),but the serum levels of other amino acids had no significant difference (P＞ 0.05).The canonical analysis showed that thirteen genes involved in ribosomal function from 9 083 genes in liver tissue in drug treatment group had the changes compared with control group (P＜ 0.05).Conclusion:Shiquandabu decoction can effectively prolong the lifetime of the TG mice by improving the levels of serum amino acids and promoting the liver ribosomal protein synthesis.关键词
十全大补汤/SV40 T抗原/氨基酸/核糖体蛋白/肝脏保护Key words
Shiquandabu decoction/SV40 T antigen/amino acid/ribosomal protein/liver protection分类
医药卫生引用本文复制引用
伍继成,赵爽,伍威,杨雷,丁晓青,郑华川..十全大补汤对SV40T抗原转基因小鼠晶状体自发肿瘤的抑制作用及其分子机制[J].吉林大学学报（医学版）,2018,44(1):13-17,后插3,6.基金项目
国家自然科学基金资助课题(81472544,81672700) （81472544,81672700）
辽宁省科技厅科技攻关项目资助课题(2015408001) （2015408001）
辽宁省教育厅科学研究基金资助课题(LJQ2014093) （LJQ2014093）
