吉林大学学报（医学版）2018，Vol.44Issue(1)：18-23,后插3,7.DOI:10.13481/j.1671-587x.20180104
穿心莲内酯对人肾细胞癌786-0细胞增殖、迁移和凋亡的影响
Effects of andrographolide in proliferation, migration and apoptosis of renal cell carcinoma 786-0 cells
摘要
Abstract
Objective:To study the inhibitory effects of andrographolide (Andro) on the proliferation,migration and clone formation ability of renal cell carcinoma (RCC) cells and the induction on the apoptosis,and to clarify their related mechanisms.Methods:The RCC cells were treated with different concentrations (5,10,20,40 and 80 μmol · L-1) of Andro as experimental groups,and 0μmol · L 1 Andro group was used as blank control group,MTS assay was used to detect the proliferation rates of RCC cells in various groups.The RCC cells were treated with different concentrations (0.50,1.25 and 2.50 μmol · L-1) of Andro as experimental groups,and 0 μmol · L 1Andro group was used as blank control group.Clonogenic assays was used to detect the colony formation ability of RCC cells in various groups.The RCC cells were treated with different concentrations (10,20and 40 μmol · L-1) of Andro as experimental groups,and 0 μmol · L-1 Andro group was used as blank control group.Wound healing assay was used to detect the migration ability of RCC cells in various groups.Flow cytometry was used to detect the apoptotic rates of RCC cells in various groups.Western blotting was performed to detect the expression levels of apoptosis related proteins in RCC cells in various groups.Results:Compared with blank control group,the proliferation rates of RCC cells in 10,20,40 and 80 μmol · L-1 Andro groups were markedly decreased (P＜0.05 or P＜0.01).Compared with blank control group,the colony formation rates of RCC cells in 0.50 and 1.25μmol · L-1 Andro groups were markedly decreased (P＜0.05 or P＜0.01).Compared with blank control group,the scratch healing rates of RCC cells in 10,20 and 40 μmol · L-1 Andro groups were markedly decreased (P＜0.01),and the apoptotic rates of RCC cells in 20 and 40 μmol · L-1 Andro groups were markedly increased (P＜0.01).Compared with blank control group,the expression level of γ-H2AX protein in 40μmol · L-1 Andro group was markedly increased (P＜0.01),the expression level of Caspase-8 protein was decreased (P＜0.05),and the expression level of cleaved Caspase-8 protein was markedly increased (P＜0.01).Conclusion:Andro can effectively suppress the proliferation,migration and colony formation ability of RCC cells and induce the apoptosis of RCC cells.The mechanism of apoptosis might be related to inducing the DNA damage and the apoptotic pathways induced by JNK / H2AX and Caspase-8.关键词
穿心莲内酯/肾肿瘤/细胞增殖/细胞迁移/细胞凋亡Key words
andrographolide/kidney neoplasms/cell proliferation/cell migration/apoptosis分类
医药卫生引用本文复制引用
毕然,杜玉君,魏伟,王春喜..穿心莲内酯对人肾细胞癌786-0细胞增殖、迁移和凋亡的影响[J].吉林大学学报（医学版）,2018,44(1):18-23,后插3,7.基金项目
国家自然科学基金资助课题(31600132) （31600132）
