吉林大学学报（医学版）2018，Vol.44Issue(1)：36-40,后插4,6.DOI:10.13481/j.1671-587x.20180107
Exendin-4对胰岛素抵抗人肝癌HepG2细胞脂代谢相关因子基因表达的影响
Effects of Exendin-4 on expressions of lipid metabolism related genes in HepG2 cells with insulin resistance
摘要
Abstract
Objective:To investigate the effects of Exendin-4 (Ex-4) on the expressions of lipid metabolism related genes in the human liver cancer HepG2 cells with insulin resistance (IR),and to elucidate the effect of Ex-4in improvement of IR.Methods:The HepG2 cells in logarithmic growth phase were induced into IR model with high concentration of insulin,then divided into control group (HepG2 cells),IR group (HepG2 cells were treated with insulin,HepG2-IR cells),and Ex-4 group (HepG2-IR cells were treated with Ex-4).Glucose oxidase (GOD-POD)kit was used to detect the consumption of glucose.The cell morphology and intracellular lipid drip formation were observed by Oil red O staining.The triglyceride (TG) level in cells was detected by kit;qRT-PCR was used to detect the mRNA expression levels of acetyl-CoA carboxylase (ACC),fatty acid synthase (FAS),sterol regulatory element-binding protein-1c (SREBP-1c) and apolipoprotein B100 (apoB100).Results:Compared with control group (HepG2 cells),the glucose consumption in the HepG2-IR cells in IR group was significantly decreased (P＜0.01).Compared with IR group,the glucose consumption in the HepG2-IR cells in Ex-4 group was increased (P＜0.05).The Oil O red staining results showed that compared with control group,the fat percentage in the HepG2-IR cells in IR group was increased (P＜0.05);compared with IR group,the fat percentage in Ex-4 group was decreased (P＜0.05).Compared with control group,the level of TG in the cells in IR group was significantly increased (P＜0.01);compared with IR group,the level of TG in the cells in Ex-4 group was significantly decreased (P＜0.05).The qT-PCR results showed that compared with control group,the expression levels of ACC FAS and SREBP-1cmRNA in the cells in IR group were increased (P＜0.01),and the expression level of apoB100 mRNA was decreased (P＜0.05);compared with IR group,the expression levels of ACC,FAS and SREBP-1c mRNA in the cells in Ex-4 group were decreased (P＜0.05),and the expression level of apoB100 mRNA was increased (P＜0.01).Conclusion:Ex-4 can regulate the expressions of lipid metabolism related genes in the HepG2 cells and improve IR.关键词
Exendin-4/HepG2细胞/胰岛素抵抗/乙酰辅酶A羧化酶/脂肪酸合成酶/脂代谢Key words
Exendin-4/HepG2 cells/insulin resistance/acetyl-CoA carboxylase/fatty acid synthase/lipid metabolism分类
医药卫生引用本文复制引用
苏寒,曲梅花,张美家,王怀杰,李娜,霍连广,李桂芝,戴功,高志芹,杨晓云..Exendin-4对胰岛素抵抗人肝癌HepG2细胞脂代谢相关因子基因表达的影响[J].吉林大学学报（医学版）,2018,44(1):36-40,后插4,6.基金项目
国家自然科学基金资助课题(31671208,H1304) （31671208,H1304）
山东省科技厅自然科学基金资助课题(ZR2015HL128,ZR2009CL047,ZR2015HL056) （ZR2015HL128,ZR2009CL047,ZR2015HL056）
山东省教育厅高等学校科技计划基金资助课题(J14LK15,J15LM10) （J14LK15,J15LM10）
