吉林大学学报（医学版）2018，Vol.44Issue(1)：41-44,后插1,后插4,6.DOI:10.13481/j.1671-587x.20180108
细胞极性相关蛋白CDC42和PAR3在小鼠牙胚发育过程中的表达
Expressions of cell polarity related proteins CDC42and PAR3 during tooth germ development in mice
摘要
Abstract
Objective:To study the expressions of cell polarity related proteins CDC42 and PAR3 during tooth germ development in the mice,and to discuss their possible roles during tooth development in the mice.Methods:The whole heads were obtained from the mouse embryo on the days 13.5,14.5,16.5 and 18.5 (E13.5,E14.5,E16.5 and E18.5) and the mice on the postnatal days 1 (PN1) and 5 (PN5).The tissues were fixed in paraformaldehyde,decalcified,dehydrated,embedded in paraffin,and sectioned.The histology of tooth germ was observed by HE staining.The expressions of CDC42 and PAR3 during tooth germ development were detected by immunohistochemistry staining.Results:The HE staining results showed that E13.5,E14.5,E16.5 and E18.5were the bud stage,the cap stage,the early and the late bell stage of tooth germ development,respectively;the tooth germ of PN1 mice showed the matured odontoblasts and ameloblasts;the tooth germ of PN5 mice showed the completed tooth crown development.The immunohistochemistry staining results showed that CDC42 expressed in the tooth germ of the mice at E13.5,E14.5 and E16.5;the CDC42 expression at E 18.5 was reduced compared with E13.5,E14.5 and E16.5;CDC42 mainly expressed in the odontoblasts and ameloblasts of the tooth germ of the mice at PN1 and PN5;PAR3 weakly expressed in the tooth germ of the mice at E13.5 and E14.5,and it was increased at E16.5 and E18.5.At PN1 and PN5,the expressions of PAR3 were decreased compared with E18.5.Conclusion:CDC42 and PAR3 partieipat in the mouse tooth development;during the early stage of tooth germ development,they may be involved in the proliferation and migration of mouse dental germ;during the late stage,CD42 and PAR3 may be involved in the differentiation of the odontoblasts and the ameloblasts,especially in the establishment and maintenance of cell polarity.关键词
细胞极性相关蛋白/CDC42/PAR3/牙胚发育/成牙本质细胞/成釉细胞Key words
cell polarity related protein/CDC42/PAR3/tooth germ development/odontoblast/ameloblast分类
医药卫生引用本文复制引用
李媛,张颖丽,孙宏晨,史册,张雪,赵欢,郝新青,胡月,刘苍维,周怡君,闫广兴..细胞极性相关蛋白CDC42和PAR3在小鼠牙胚发育过程中的表达[J].吉林大学学报（医学版）,2018,44(1):41-44,后插1,后插4,6.基金项目
国家重点研发专项基金资助课题(2016YFC1102800) （2016YFC1102800）
国家自然科学基金国际(地区)合作与交流项目资助课题(81320108011) （地区）
国家自然科学基金青年科学基金资助课题(81600843) （81600843）
吉林省卫计委项目资助课题(2016Q025) （2016Q025）
吉林省科技厅科技发展计划优秀青年人才基金项目资助课题(20170520016JH) （20170520016JH）
