重庆医学2018，Vol.47Issue(7)：941-943,3.DOI:10.3969/j.issn.1671-8348.2018.07.022
基于Illumina Miseq平台分析胆囊结石及黏膜微生物群落的多样性
Analysis on microbial community diversity in gallbladder stone and mucosa based on Illumina Miseq platform
摘要
Abstract
Objective To investigate the microbial community diversity in stone and mucosa from the patients with gallbladder stone.Methods The gall bladder mucosal samples in 60 cases(case group) of gallbladder stone treated by cholecystectomy amd 11 cases (control group)of liver right anterior lobe hemangioma in this hospital from January to December 2014 were selected for conducting the bacterial culture.The stone and mucosa in the patients with negative culture results were performed the Illumina Miseq sequencing analysis.Results A total of 71 cases were included in the study.The microbial community DNA in the control group samples was not detected out.The bacterial DNA detection rate of samples in the case group was 94.2％;in regardless of the type of sample,the most dominant was Phylum Proteobacteria.The composition proportion of stone and mucosal microbial community in the patients with same type of stone was relatively consistent,whereas different types of composition had large difference.Conclusion Using the Illumina Miseq sequencing analysis can effectively evaluate the gallbladder microbial community diversity in the patients with gallbladder stone.关键词
胆囊结石/黏膜/微生物/Illumina Miseq测序Key words
gallbladder stones/mucosa/microorganism/Illumina Miseq sequencing分类
医药卫生引用本文复制引用
黄洁,张捷..基于Illumina Miseq平台分析胆囊结石及黏膜微生物群落的多样性[J].重庆医学,2018,47(7):941-943,3.基金项目
云南省卫生和计划生育委员会内设机构研究基金项目(2014NS060). （2014NS060）
