分析测试学报2017，Vol.36Issue(12)：1417-1423,7.DOI:10.3969/j.issn.1004-4957.2017.12.001
基于超高效液相色谱-质谱的胶质瘤患者血浆代谢组学研究
Study on Plasma Metabolomics of Glioma Patients by Ultra Performance Liquid Chromatography-Mass Spectrometry
摘要
Abstract
In this study,the ultra high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap-HRMS) was firstly applied in the analysis of the plasma from the glioma patients and the normal control group to find the differential metabolites,which could provide the scientific evidence for clinical diagnosis and pathogenesis research.The spectra obtained by UHPLC-Q-Orbitrap HRMS were conducted to peak identification,peak matching and denoising.The data of metabolomics were statistically analyzed by using principal component analysis and orthogonal partial least-squares-discriminant analysis method to screen the differential metabolites with VIP ＞ 1.0 and P ＜ 0.05,which were investigated to further evaluate the diagnostic ability.The results showed that the plasma metabolic profiles of glioma patients were dramatically altered,and ten potential biomarkers were identified.The metabolites of leucine,valine,tryptophan,choline and taurine were significantly reduced in glioma patients,while histidine,citric acid,lactic acid,creatine and pyruvic acid were obviously increased compared with the normal controls (P ＜ 0.05),which suggested that amino acid and energy metabolism might play an important role in the development of glioma.In addition,the different metabolites showed good diagnostic ability toward gliomas (AUC ＞ 0.8),which could be used as potential diagnostic biomarkers.关键词
胶质瘤/超高效液相色谱-四极杆-静电场轨道阱质谱技术/代谢组学/诊断标志物Key words
glioma/ultra high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry/metabolomics/diagnosis biomarker分类
化学化工引用本文复制引用
左莉华,张晓坚,丁大领,沈燕,师莹莹,孙志,李卓伦,周霖,徐昙烨,刘丽伟..基于超高效液相色谱-质谱的胶质瘤患者血浆代谢组学研究[J].分析测试学报,2017,36(12):1417-1423,7.基金项目
北京医卫健康公益基金会医学科学研究基金(YWJKJJHKYII-B17819) （YWJKJJHKYII-B17819）
