分析测试学报2017，Vol.36Issue(12)：1431-1438,8.DOI:10.3969/j.issn.1004-4957.2017.12.003
QuEChERS结合超高效液相色谱-串联质谱法同时测定番茄中噻虫嗪、噻虫胺、螺虫乙酯及其代谢物残留
Analysis of Thiamethoxam, Clothianidin and Spirotetramat and Their Metabolites Residues in Tomato Using QuEChERS Method with Ultra Performance Liquid Chromatography-Mass Spectrometry
摘要
Abstract
In this study,an analytical method was established for the determination of thiamethoxam and its metabolites clothianidin,spirotetramat and its four metabolites such as BYI08330-enol-glucoside,BYI08330-ketohydroxy,BYI08330-enol and BYI08330-mono-hydroxy residues in tomatoes using QuEChERS cleanup with ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The residues in samples were extracted with acetonitrile,dehydrated with anhydrous magnesium sulfate and sodium chloride,then purified with primary secondary amine (PSA) sorbent and C18,finally detected by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) under the multi-reactive ion monitoring mode (MRM) and quantified by external standard method.The results showed that good linearities for the seven analytes i.e.thiamethoxam,clothianidin,spirotetramat,BYI08330-enol-glucoside,BYI08330-mono-hydroxy,BYI08330-enol and BYI08330-ketohydroxy were observed in the concentration range of 0.2-2 000 μg/L with the correlation coefficients(r2) not less than 0.999 2.The limits of quantification(LOQs) were 0.002 mg/kg.The average recoveries at the fortified levels of 0.002,0.02,0.20 and 2.O0 mg/kg were in the range of 79.9％-104％ with the relative standard deviations of O.94％-6.4％.With the advantages of rapidness,simplicity,high sensitivity and good reproducibility,the method is suitable for the determination of thiamethoxam,clothianidin,spirotetramat and their four metabolites residues in tomatoes.关键词
QuEChERS法/超高效液相色谱-串联质谱法/农药残留/番茄Key words
QuEChERS/UPLC-MS/MS/pesticide residues/tomato分类
化学化工引用本文复制引用
刘炎,欧阳迪庆,叶玉凤,王成秋,焦必宁,赵其阳,张耀海,敖艳,王海燕..QuEChERS结合超高效液相色谱-串联质谱法同时测定番茄中噻虫嗪、噻虫胺、螺虫乙酯及其代谢物残留[J].分析测试学报,2017,36(12):1431-1438,8.基金项目
2017年国家柑桔及热带作物产品质量安全风险评估项目(GJFP2017004) （GJFP2017004）
国家现代农业(柑桔)产业技术体系建设项目(CARS-26) （柑桔）
