工业微生物2017，Vol.47Issue(6)：14-18,5.DOI:10.3969/j.issn.1001-6678.2017.06.003
富硒长双歧杆菌总蛋白对黑色素瘤细胞活性研究
Activity studies of whole protein in selenium enriched Bifidobacterium longum on melanoma cells
摘要
Abstract
The whole protein of Bifidobacterium longum was used as reference to investigate the inhibitory effects of whole protein in selenium enriched Bifidobacterium longum on melanoma cells.The results showed that when the total protein concentration of selenium enriched Bifidobacterium longum reached 100 μg/mL,its inhibitory effects on melanoma cells were significantly higher than those of the whole protein of Bifidobacterium longum and positive control drug 5-FU.Meanwhile,whole protein of selenium enriched Bifidobacterium longum combined with 5-FU had synergistic inhibition on melanoma cells.Furthermore,the melanoma intracellular ROS levels were determined by using reactive oxygen test kit.The results indicated that the melanoma cells treated with whole protein of selenium enriched Bifidobacterium longum could be significantly resistant to the intracellular ROS surge induced by Rosup.关键词
富硒长双歧杆菌/黑色素瘤细胞/抑制作用Key words
Selenium enriched Bifidobacterium longum/melanoma cell/inhibition引用本文复制引用
张素姬,刘海燕,陈代杰,谭俊,王旻,邵雷..富硒长双歧杆菌总蛋白对黑色素瘤细胞活性研究[J].工业微生物,2017,47(6):14-18,5.基金项目
国家“重大新药创制”科技重大专项(No:2013ZX09301302,No.2014ZX09507009-025) （No:2013ZX09301302,No.2014ZX09507009-025）
国家自然科学基金(No.81573329,No.81773616) （No.81573329,No.81773616）
国家青年科学基金(No.81603004) （No.81603004）
上海市优秀技术带头人计划(17XD1423200) （17XD1423200）
上海市自然科学基金(16ZR1435400). （16ZR1435400）
