工业微生物2017，Vol.47Issue(6)：38-42,5.DOI:10.3969/j.issn.1001-6678.2017.06.007
乳酸乳球菌冷冻保藏条件的研究
Research on cryopreservation conditions of Lactococuus lactis
摘要
Abstract
Lactococcus lactis exerts health benefits,inhibiting the growth of harmful bacteria,protecting the gut and enhancing human health.But in the practical application there is a big problem,which is difficult to preserve their activity and the activity is relatively low,especially in cryopreservation.In this paper,the perfect protective agent combination was determined by single factor and orthogonal experiment,including 30％ trehalose,10％ glycerol,20％ sucrose,30％ skimmed milk.Using this composite preservative agent,the survival rate of L.lactis after frozen could reach 91.96％.Furthermore,the effects of frozen cooling rate and frozen preservation after different thawing methods on the survival rate of L.lactis were investigated.The results revealed that the best cooling rate was-20 ℃/min and the best thawing temperature was 0 ℃.Under the above conditions,the survival rate was 93.14％.关键词
乳酸乳球菌/冷冻保藏/保护剂/降温速率/解冻温度Key words
Lactococcus lactis/cryopreservation/protective agent/cooling rate/thawing method引用本文复制引用
王光强,于小青,周炜,郭柏松,尚禹,艾连中,张汇..乳酸乳球菌冷冻保藏条件的研究[J].工业微生物,2017,47(6):38-42,5.基金项目
2016年度闵行区产学研合作计划项目. （）
