华北农学报2018，Vol.33Issue(1)：1-6,6.DOI:10.7668/hbnxb.2018.01.001
水稻肽链释放因子OseRF1-3基因克隆、表达及启动子分析
Cloning,Expression and Promoter Analysis of Polypeptide Release Factor OseRF1-3 Gene from Rice
摘要
Abstract
To reveal the function of rice polypeptide release factor eRF1 in protein synthesis,the full length cD-NA and promoter (2 120 bp) of rice eRF1 gene,named OseRF1-3,was isolated by PCR.Sequence analysis indica-ted that the OseRF1-3 contained a 1 308 bp CDS sequence that encoded 436 amino acids with three conserved N, M and C domains.qRT-PCR results indicated that the transcript of OseRF1-3 was detected in all of the tissues ex-amined,but the highest level of expression was detected in spikes.The genome DNA from leaves of rice was used as the template,the 2 120 bp promoter sequence of the initiation codon upstream of OseRF1-3 gene were cloned using the PCR method. The promoter of OseRF1-3 gene was fused with GUS reporter gene and transferred into Oryza sati-va L.callus by Agrobacterium-mediated method.Histochemical staining of different organs of the transgenic plants showed that histochemical GUS expression could be found in all of the tissues examined,and a low GUS reporter gene expression in root and higher expression in flowers and rice husk.The results would provide the basis for the understanding of the OseRF1-3 gene function. The results of GUS gene expression driven by the promoter of OseRF1-3 gene were in good agreement with the results from qRT-PCR analysis. So the results will provide the ba-sis for the further explore of the OseRF1-3 gene function.关键词
水稻/肽链释放因子/启动子/表达模式/GUSKey words
Rice/Polypeptide release factor/Promoter/Expression pattern/GUS分类
农业科技引用本文复制引用
唐跃辉,乔蓉,李成伟,包欣欣,刘坤,张慧聪,王双,赵君苇,娄慧敏,王箐,梁静..水稻肽链释放因子OseRF1-3基因克隆、表达及启动子分析[J].华北农学报,2018,33(1):1-6,6.基金项目
2018年度河南省高等学校重点科研项目(18A180035) （18A180035）
河南省科技开放合作项目(132106000077) （132106000077）
河南省科学技术厅科技攻关项目(152102410074) （152102410074）
周口师范学院青年基金(ZKNUB3201801) （ZKNUB3201801）
高层次人才基金(ZKNUC2016030) （ZKNUC2016030）
