华北农学报2018，Vol.33Issue(1)：33-38,6.DOI:10.7668/hbnxb.2018.01.006
家蚕抗菌肽基因的克隆及重组蛋白的自诱导表达
Cloning of Antibacterial Peptide Gene and Auto-induction Expressing of Recombinant Proteins from Bombyx mori
摘要
Abstract
Antibacterial peptide is a kind of small molecular compound with antibacterial activity in organisms, especially in insect cells that resist the infection of foreign microbe,to research cloning of antibacterial peptide genes and expressing and purification of recombinant proteins from Bombyx mori. Four pairs of special primers were de-signed,and four genes BmCecropin,BmCecropinB6,BmCecropinD and Bmmoricin were amplified by RT-PCR from total RNA extracted from midgut from silkworm,then four recombinant proteins were expressed and purified by auto-induc-tion and Ni-NTA affinity chromatography and ultrafiltration purification.The results showed that four antibacterial pep-tide genes were 198,108,105,129 bp in sizes,respectively.Further SDS-PAGE electrophoresis to detect four recombi-nant antibacterial peptide,results showed that four recombinant antibacterial peptide by auto-induction expressing were 24,21,20,22 ku in sizes,respectively.The four purified antibacterial peptides constructed a basis on subse-quent antibacterial test and further application on bacteriostatic agent,cosmetics and preservatives.关键词
家蚕/抗菌肽/重组蛋白/自诱导表达/纯化Key words
Bombyx mori/Antibacterial peptide/Recombinant protein/Auto-induction expressing/Purified分类
生物科学引用本文复制引用
谢昆,王靖,王丽仙,朱灵明,尹建华,叶青霞,孙艳..家蚕抗菌肽基因的克隆及重组蛋白的自诱导表达[J].华北农学报,2018,33(1):33-38,6.基金项目
云南省大学生创新创业训练计划项目(DCXM163018) （DCXM163018）
红河学院应用型科学研究项目(XJY15Z07) （XJY15Z07）
红河学院博士专项(XJ15B13) （XJ15B13）
云南省昆虫生物医药研发重点实验室开放课题 （）
