华北农学报2018，Vol.33Issue(1)：39-44,6.DOI:10.7668/hbnxb.2018.01.007
水稻OsGPRP家族基因克隆及其对非生物胁迫的响应
Cloning of OsGPRP Family Genes and Their Responses to Abiotic Stresses in Rice
摘要
Abstract
Genes encoding glycine-and proline-rich proteins (GPRPs) are widely distributed in higher plants, playing important roles in plant growth and development,and stress adaptation. In this study,to clone rice OsG-PRPs,bioinformatics analysis combining RT-PCR and sequencing were performed.Complementary DNA (cDNA) sequences of three OsGPRPs encoding OsGPRPs with 197,180 and 170 amino acids,respectively,were obtained. Each OsGPRP encoded contained three motifs typical in plant GPRPs including an N-terminal XYPP repeat,a cen-tral hydrophobic region rich in alanine residues and a C-terminal HGK repeat.Online cis-acting regulatory element analysis against PlantCARE database were used to explore the function of these OsGPRPs.Plenty of putative cis-ele-ments related to plant growth and hormone and stress responses were identified in the promoters of these OsGPRPs. To further understand the biological function of these OsGPRPs during the process of abiotic stress adaptation,quan-titative real-time PCR (qRT-PCR) were used to analyze their responses under drought and cold stresses in rice seedlings.Responses of all three OsGPRPs to both drought and cold stresses were detected.Similar expression pat-terns with up-regulation of these OsGPRPs in response to drought were shown in different rice seedling tissues.How-ever,obviously different patterns in response to low temperature were shown with elevated OsGPRP1 and OsGPRP3 but reduced OsGPRP2 expression levels.Results indicate important adaptive roles of these three OsGPRPs to abiotic stresses in rice.关键词
水稻/OsGPRP/基因克隆/非生物胁迫/基因表达Key words
Rice/OsGPRP/Gene cloning/Abiotic stress/Gene expression分类
生物科学引用本文复制引用
吴蔚蔚,童普国,王鑫,阎新,李绍波,欧阳解秀..水稻OsGPRP家族基因克隆及其对非生物胁迫的响应[J].华北农学报,2018,33(1):39-44,6.基金项目
国家自然科学基金项目(31560298) （31560298）
