华北农学报2018，Vol.33Issue(1)：224-232,9.DOI:10.7668/hbnxb.2018.01.032
生物炭不同施用量对烟草根际土壤微生物多样性的影响
Effects of Different Biochar Application Rates on Rhizosphere Soil Microbial Diversity of Tobacco
摘要
Abstract
In order to study the influence of different application amounts of charcoal on the microbial diversity in tobacco rhizosphere soil,with the rhizosphere soil of flue-cured tobacco variety Yunyan 87 as the research ob-ject,we compared the situation by applying different amounts of charcoal,and conducted high-throughput sequen-cing of rhizosphere soil ITS2 region and 16S rDNA-V4 region with progressive treatment with 3 application amounts of charcoal; through bioinformatics analysis of data, we obtained the OTU abundance, distribution,α diversity, community species composition and abundance information of microorganisms in rhizosphere soil when different treatment methods were used,and conducted PCA clustering analysis and UPGMA clustering analysis of the com-munity composition and abundance. The results showed that within the application scope of 50-150 g/tree,by in-creasing the application amounts of charcoal, it could increase the variety diversity and distribution uniformity of bacteria in rhizosphere soil,while it would reduce the variety diversity and distribution uniformity of fungus;com-pared to the control group where no charcoal was applied,after applying the charcoal with the amounts of 50,100, 150 g/tree,the abundances of Proteobacteria bacteria had declined by 4.1%,2.7% and 0.7% respectively;the abundances of Acidobacteria bacteria increased by 10.4%,8.1% and 7.7% respectively;the abundances of Acti-nobacteria bacteria were all lower than the abundance of control group;the difference in abundance of Gemmatimo-nadetes bacteria between various groups was very small. After applying the charcoal with the amount of 50 g/tree, the abundance of Zygomycota fungus had declined by 12.38% compared to the control group(50.92%), and then,with the increased of the application amount of charcoal, the abundance of Zygomycota fungus gradually in-creased to 53.68%;on the contrary,after applying the charcoal with the amount of 50 g/tree, the abundance of Ascomycota fungus had increased by 10.15% compared to the control group(30.63%), and then, with the in-creased of the application amount of charcoal,the abundance of Ascomycota fungus gradually declined to 29.11%;after applying the charcoal with the amount of 50 g/tree, the abundances of Basidiomycota and Chytridiomycota funguses almost had no change,and then,with the increased of the application amount of charcoal,the abundances of Basidiomycota and Chytridiomycota funguses presented the trend of increased first, and then declined; various treatment methods did not have significant influence on the abundance of Glomeromycota fungus. By studying the influencing method and action rules of charcoal application on the micro-ecology of tobacco rhizosphere,it can pro-vide theoretical basis to the application of charcoal in tobacco field.关键词
生物炭/烟草/根际土壤/微生物/多样性Key words
Biochars/Tobacco/Rhizosphere soil/Microbial/Diversity分类
生物科学引用本文复制引用
陈泽斌,高熹,王定斌,郭丽红,王定康,徐胜光..生物炭不同施用量对烟草根际土壤微生物多样性的影响[J].华北农学报,2018,33(1):224-232,9.基金项目
国家自然科学基金项目(41361056) （41361056）
昆明学院"人才引进项目"(YJL14005) （YJL14005）
云南省高校优势特色重点学科(生态学)建设项目(05000511311) （生态学）
云南省特色生物资源开发与利用重点实验室开放基金项目(GXKJ201621) （GXKJ201621）
