河北医学2018，Vol.24Issue(2)：177-181,5.DOI:10.3969/j.issn.1006-6233.2018.02.001
Rho相关激酶在高糖诱导小鼠心脏内皮细胞衰老中的作用研究
Effects of Rho-Associated Kinase on High Glucose-Induced Murine Heart Endothelial Cell Senescence
摘要
Abstract
Objective:To investigate the role of Rho-associated kinase in the high glucose-induced mu-rine heart endothelial cells (MHECs) senescence. Methods: MHECs senescence was induced by culturing with 22 mM glucose for 72 h. MHECs in control group were treated with normal culture media and the MHECs in High glucose (HG) group were treated with 22 mM glucose. MHECs in Y27632 group were pretreated with Rho-associated kinase inhibitor Y27632 before cultured in high glucose. In addition, mannitol was added to exclude the effect of osmotic pressure.The ratio of senescent cells was identified by senescence-associated β-galactosidase (SA-βG) staining and flow cytometry was used to analyze the cell cycle. The expression of se-nescence associated protein p21, p-MBS(Thr853) and MBS was explored by Western blot. The telomerase activity in endothelial cells and the level of nitric oxide(NO) in culture medium assay kit were detected with respective kits. Results:Compared to the control group,treatment of high glucose could significantly increase the ratio of SA-βG positive cells(44.00%±3.63%vs.4.17%±0.70%,P<0.01),enhance ROCK activity,in-crease the ratio of cells in G0/G1 stage and the expression of p21,inhibit telomerase activity of the cells and NO level in culture medium (all P<0.05). However, treatment of ROCK inhibitor Y27632 could greatly in-hibit ROCK activity and effectively decrease the ratio of senescent cells (both P<0.05). Also, treatment of Y27632 could decrease the ratio of cells in G0/G1 stage and the expression of p21 and reverse the decrease of telomerase activity and NO level in cells cultured in high glucose medium(all P<0.05). Conclusion:Rho re-lated kinase activation is involved in high glucose induced endothelial cell senescence, and inhibition of Rho related kinase can reduce the senescence of endothelial cells induced by high glucose,and provide a new di-rection for the prevention and treatment of diabetic angiopathy.关键词
Rho相关激酶/细胞衰老/高糖/内皮细胞Key words
Rho-associated kinase/Cellular senescence/High glucose/Endothelial cell引用本文复制引用
徐昌武,邱立强,江洪..Rho相关激酶在高糖诱导小鼠心脏内皮细胞衰老中的作用研究[J].河北医学,2018,24(2):177-181,5.基金项目
国家自然科学基金,(编号:81500228) （编号:81500228）
