河南农业大学学报2018，Vol.52Issue(1)：1-5,5.
玉米单倍体成熟胚组培加倍效果研究
Study on doubling effect of mature haploid embryo based on tissue culture in maize
摘要
Abstract
In this study,the haploid embryos from mature kernels of Xianyu335 induced by Yu High Inducer No.1(YHI-1),were treated with 0.005%,0.01%,0.02% and 0.03% colchicines for 12 hours,24 hours and 36 hours,respectively,with MS medium without colchicine as the control, to in-vestigate the plantlet rate and the doubling rate of the haploid plant. The results showed that the higher the quality volume fraction of colchicine and the longer the treatment time was, the lower the plantlet rate was. The highest plantlet rate reached 96.97% when the haploid embryos were treated with 0.005% quality volume fraction of colchicine for 12 hours, while the treatment of 0.03% with 36 hours,the lowest plantlet rate was the only 24.23% when the haploid embryos were treated with 0.03% quality volume fractiron of colchicine for 36 hours. The interaction between the treatment time and quality volume fraction of colchicine existed significantly. The doubling rate of haploid plants in-creased with the higher quality volume fraction of colchicine in a short period of time(12 hours),but decreased with the increase of treatment time at higher quality volume fraction(more than 0.01%) of colchicine. Considering the plantlet rate and doubling rate,the treatment of 0.005% colchicine for 24 hours is the best,and the comprehensive efficiency could reach as high as 57.61%.关键词
玉米/成熟胚/组织培养/单倍体加倍Key words
maize/mature embryo/tissue culture/haploid doubling分类
农业科技引用本文复制引用
李浩川,李颜,曲彦志,杨继伟,张朝林,王盼盼,崔丽洋,毛熙岚,刘宗华..玉米单倍体成熟胚组培加倍效果研究[J].河南农业大学学报,2018,52(1):1-5,5.基金项目
国家自然科学基金项目(U1204316) （U1204316）
国家十三五重点研发计划项目(2016YFD0101205-3) （2016YFD0101205-3）
河南省科技攻关项目(162102110014) （162102110014）
