河南农业大学学报2018，Vol.52Issue(1)：59-65,7.
内生细菌EBS05对番茄植物的促生和诱导抗病性信号转导途径的研究
Study on growth promotion and signaling pathway of induced systemic resistance elicitation mediated by endophytic bacteria strain EBS05 in tomato plant
摘要
Abstract
The plant growth promotion and signaling pathway of induced systemic resistance(ISR) elicitation mediated by endophytic bacteria strain EBS05 in tomato plant against tomato yellow leaf curl virus (TYLCV) were investigated using the tomato variety Jiangsu 14 as a test material. The results showed that strain EBS05 could significantly promote the growth of tomato plants by inducing the over-expression of LeEXP2,LeEXP5 and LeEXP18 genes with related to cell growth in tomato plant. The key regulatory genes NPR1 and PR1 in salicylic acid (SA)-signaling transduction pathway in test plants pretreated by strain EBS05 suspension were activated at the first day after inoculated with TYL-CV and over-expressed from the third day to the fifth day,and reached maximum expression at the sev-enth day. The expression levels increased sixteen fold and thirteen fold compared with those of genes in control plants. It suggested that ISR mediated by strain EBS05 in tomato plant against TYLCV depen-ded on SA-signaling transduction pathway. Meanwhile,the gene Coi1,as a key regulatory gene in jas-monic acid (JA)-signaling transduction pathway,was elicited and over-expressed at the fifth day after inoculated with TYLCV. It suggested that cross-talk between SA-and JA-dependent signaling transduc-tion pathways might be involved in ISR mediated by strain EBS05 in tomato plants defense against TYLCV.关键词
内生细菌/促生作用/番茄黄化曲叶病毒/系统诱导抗性/信号转导途径Key words
endophytic bacteria/plant growth promotion/tomato yellow leaf curl virus/induced sys-temic resistance/signaling pathway分类
农业科技引用本文复制引用
赵玉华,李俊州,冯慧静,文才艺..内生细菌EBS05对番茄植物的促生和诱导抗病性信号转导途径的研究[J].河南农业大学学报,2018,52(1):59-65,7.基金项目
国家自然科学基金项目(31272083) （31272083）
河南省教育厅科学技术研究重点项目(13A210473) （13A210473）
