环境工程学报2018，Vol.12Issue(1)：190-197,8.DOI:10.12030/j.cjee.201704016
不同植物-微生物联合修复体系下石油烃的降解
Degradation of petroleum hydrocarbons under different plant-microbial remediation system
摘要
Abstract
As one of the organic pollutants widely present in the environment,petroleum hydrocarbon is harmful to human health.Low temperature-resistance and high efficient petroleum hydrocarbon degrading bacteria screened from crude oil contaminated soil in Dagang Oil Field was selected as target cells.Wheat and alfalfa were used as tested plants.The effects of different plant types and different inoculation methods on oil degradation were compared.We used fluorescein assay to analyze fluorescein acetate (FDA) enzyme activity changes with time.After 70 days of degradation,the total petroleum hydrocarbon content of undisturbed soil sample decreased from 30 720 mg · kg-1 to 26 800 mg · kg-1,the degradation rate was up to 12.76％.Compared with wheat,alfalfa had a better effect on the degradation of petroleum hydrocarbons,the degradation rate was 24.39％.Under the condition of wheat immobilized bacteria,the degradation rate was 21.10％,and the degradation rate of petroleum hydrocarbon was much higher than that of other treatments in the later stage,which showed good potential for recovery.The activity of FDA was decreased first,then increased and then decreased until it was stable,and affected by both plant and exogenous bacteria.关键词
石油烃/小麦/紫花苜蓿/菌悬液/固定化外源菌/FDA酶活性Key words
petroleum hydrocarbon/wheat/alfalfa/bacterial suspension/immobilized bacteria/FDA enzyme activity分类
资源环境引用本文复制引用
刘自力,王红旗,孔德康,许洁,吴枭雄,熊樱..不同植物-微生物联合修复体系下石油烃的降解[J].环境工程学报,2018,12(1):190-197,8.基金项目
国家高技术研究发展计划(863)项目(2013AA06A205) （863）
