河南农业科学2017，Vol.46Issue(11)：30-34,5.DOI:10.15933/j.cnki.1004-3268.2017.11.006
高压电场处理对高粱种子萌发期酶活性的影响
Effects of High Voltage Electric Field on Enzyme Activity of Sorghum Seeds during the Germinating Period
摘要
Abstract
In order to explore the reason why appropriate high voltage electric field(HVEF) can promote sorghum seed germination,the experiment was designed to decipher the possible mechanism.This experiment used the Jinza 122 as test material and the completely random design of experiment as method,and mainly focused on the change of αx-amylase,CAT,SOD,POD activity of sorghum seeds.The results showed that the seed vigor of sorghum seeds increased with the treatment of HVEF.Further study showed that when the electrostatic field was 500 kV/m and the treatment time was 30 min (T4),the germination comprehensive index value reached maximum,followed by T5 (500 kV/m × 40 min).Compared to the control group (not treated by HVEF),all test groups showed higher α-amylase activity and among them T4 showed the highest α-amylase activity,which was 3.80 mg/(g · min) and increased by 156.75％ compared to the control group.The CAT activity of all the test groups decreased except T2 (400 kV/m × 40 min),T5,T8 (600 kV/m × 40 min).All POD activities of test groups were higher than control group except T8,which was similar to the control group.T3 showed the highest POD activity,which was 115.81 U/(g · min) and increased by 69.16％ compared to the control group.The SOD activities of all the test groups were higher than that of control,and T4 reached maximum value,which was 774.45 U/g and increased by 175.31％,followed by T2 and T5,which were increased by 171.93％ and 100.05％.The results indicate that appropriate HVEF can promote sorghum seed germination by increasing the α-amylase activity and protective enzyme activity.关键词
高压电场/高粱/种子萌发/α-淀粉酶/CAT/SOD/PODKey words
high voltage electric field/sorghum/seed germination/α-amylase/CAT/SOD/POD分类
农业科技引用本文复制引用
胡建芳,张作伟,杜慧玲,陈建中,王慧杰..高压电场处理对高粱种子萌发期酶活性的影响[J].河南农业科学,2017,46(11):30-34,5.基金项目
山西省农业科技攻关项目(20130311014-3) （20130311014-3）
