河南农业科学2017，Vol.46Issue(11)：103-107,5.DOI:10.15933/j.cnki.1004-3268.2017.11.018
皂荚种质资源RSAP遗传多样性分析及指纹图谱的构建
Genetic Diversity and Fingerprints of Glediysia sinensis Germplasms Based on RSAP
摘要
Abstract
Genetic diversity of 18 Glediysia sinensis germplasms was analyzed by the RSAP markers.The results showed that 12 primers were screened out of 45 primer pairs and 167 bands were obtained,including 154 polymorphic bands,with the percentage of polymorphic loci(PPL) of 92.22％.The average Nei's gene diversity(H) and Shannon's information index(Ⅰ) were 0.229 8 and 0.371 6,and the genetic similarity coefficients among the tested samples ranged from 0.431 1 to 0.988 0.UPGMA analysis showed that 18 Glediysia sinensis were clustered into 3 groups with the genetic similarity coefficient of 0.62.Glediysia heterophylla formed the first group,Glediysia melanacantha and Zaojia-T were clustered into the second group,and the others were clustered into the third group.In total of 18 Glediysia sinensis germplasm DNA fingerprints were constructed from 9 polymorphic loci amplified by 6 primer pairs and these materials could be distinguished and identified accurately.关键词
皂荚/RSAP/DNA指纹图谱/遗传多样性Key words
Glediysia sinensis/RSAP/DNA fingerprint/genetic diversity分类
农业科技引用本文复制引用
范定臣,张安世,刘莹,骆扬..皂荚种质资源RSAP遗传多样性分析及指纹图谱的构建[J].河南农业科学,2017,46(11):103-107,5.基金项目
河南省科技攻关项目(14210221100850) （14210221100850）
2015年河南省林业厅科技兴林项目 （）
