河南农业科学2017，Vol.46Issue(11)：138-143,6.DOI:10.15933/j.cnki.1004-3268.2017.11.024
甘加藏羊发情周期不同阶段血浆生殖激素分泌规律
Dynamics Research of Reproductive Hormone Secretion in the Plasma of Ganjia Tibetan Sheep in Different Stages of Estrous Cycle
摘要
Abstract
To study the secretion changes of reproductive hormones in Ganjia Tibetan sheep estrous cycle in plasma,the blood of different estrous cycle stages from 24 Ganjia Tibetan sheep was collected and the secretion dynamic change of follicle-stimulating hormone (FSH),luteinizing hormone (LH),progesterone (P) and estrogen (E) in plasma of the Tibetan sheep estrous cycle was tested by enzyme-linked immunoassay(ELISA).The results showed that content of FSH was the highest(3.406 mIU/mL)in the estrus,then reduced to 2.492 mIU/mL in the late estrus and began to rise in the dioestrus,and achieved 2.727 mIU/mL in the proestrus.The content of FSH in the estrus was significantly higher than that of the late estrus,dioestrus and proestrus.The content of LH in the dioestrus was the highest (4.377 mIU/mL),and the late estrus was significantly lower than dioestrus and proestrus.The content of P was the highest (18.039 ng/mL) in the proestrus,then reduced to 16.196 ng/mL in the estrus and rose slowly in the late estrus (achieved to 17.127 ng/mL in the dioestrus).The content of P in the proestrus was significantly higher than that of the estrus and the late estrus.The content of E was the lowest (29.625 pg/mL) in proestrus,rose to 36.035 pg/mL in the estrus and achieved to the highest in the late estrus(38.573 pg/mL),then reduced to 35.531 pg/mL in the dioestrus.The content of E in the late estrus was significantly higher than the estrus,dioestrus and proestrus,and that of proestrus was significantly lower than dioestrus and estrus.关键词
甘加藏羊/发情周期/促卵泡素/促黄体素/孕酮/雌激素/ELISAKey words
Ganjia Tibetan sheep/estrous cycle/FSH/LH/P/E/ELISA分类
农业科技引用本文复制引用
朱雪雁,葛闻博,何玉琴,史军红,王文芳..甘加藏羊发情周期不同阶段血浆生殖激素分泌规律[J].河南农业科学,2017,46(11):138-143,6.基金项目
国家自然科学基金项目(31360595) （31360595）
