河南农业科学2017，Vol.46Issue(11)：148-151,4.DOI:10.15933/j.cnki.1004-3268.2017.11.026
猪肺炎支原体与猪鼻支原体分离鉴定及鉴别检测
Identification and Differential Diagnosis of Mycoplasma hyosynoviae and Mycoplasma hyorhinis
摘要
Abstract
In order to develop differential diagnosis method of Mycoplasma hyosynoviae and Mycoplasma hyorhinis,Mycoplasma was isolated and identified from Henan province and surrounding areas.Two pairs of primers were designed to amplify Mycoplasma hyosynoviae and Mycoplasma hyorhinis DNA and the fragment sizes were 480 bp and 360 bp respectively.The results showed that the colony was a typical "fried egg" characteristic.The single amplified PCR showed that the isolated microbes were Mycoplasma hyosynoviae and Mycoplasma hyorhinis.On the basis of single amplification,the double PCR reaction was established for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis.The double PCR method could be used for detection and differential diagnosis of Mycoplasma hyosynoviae and Mycoplasma hyorhinis.关键词
猪肺炎支原体/猪鼻支原体/鉴别诊断/二重PCRKey words
Mycoplasma hyosynoviae/Mycoplasma hyorhinis/differential diagnosis/double PCR分类
农业科技引用本文复制引用
李海利,郎利敏,王克领,邓祖丽颖,徐引弟,王治方,张青娴,朱文豪,方剑玉,游一,侯自花..猪肺炎支原体与猪鼻支原体分离鉴定及鉴别检测[J].河南农业科学,2017,46(11):148-151,4.基金项目
河南省农业科学院自主创新基金项目(2016ZC49) （2016ZC49）
