核农学报2018，Vol.32Issue(1)：40-47,8.DOI:10.11869/j.issn.100-8551.2018.01.0040
叶脉分化发育关键基因COV1在柠条锦鸡儿中的克隆和表达分析
Cloning and Expression Analysis of COV1, a Key Gene in the Differentiation and Development, From Caragana korshinskii Kom.
摘要
Abstract
COV1 is a key gene in leaf vein development.To explore the vital function of COV1 in regulating the development of Caragana korshinskii Kom.vascular system,especially in the development of veindifferentiation under the increasing drought,a COV1 encoding gene fragment was isolated from transcriptome sequencing database of Caragana korshinskii Kom.,and the parthal length cDNA was cloned by RT-PCR.Sequencing results showed that the parthal cDNA length was 946 bp,with an open reading frame of 777 bp.It encoded a protein composed of 258 amino acids.The putative protein molecular weight was 28.84 KD,and isoelectric point was 5.46.The deduced protein is strongly hydrophobic.Bioinformatics analysis showed that the COV1 gene in Caragana korshinskii Kom.was most closed to COV1 in the leguminous model plant Medicago truncatula Kom..RT-qPCR analysis showed that the transcript of COV1 was inhibited under drought stress.These results indicated that the suppression of COV1 gene expression under drought stvess is associated possibly with vascular system development.This further clarifying molecular mechanism of developed vascular system in Caragana korshinskii Kom.under the drought stress.关键词
COV1/柠条锦鸡儿/干旱胁迫/维管系统Key words
COV1/Caragana korshinskii Kom./drought stress/vascular system引用本文复制引用
高丽芳,孙莹莹,周文菲,白娟,龚春梅..叶脉分化发育关键基因COV1在柠条锦鸡儿中的克隆和表达分析[J].核农学报,2018,32(1):40-47,8.基金项目
国家自然科学基金面上项目(31070538、31370599) （31070538、31370599）
