核农学报2018，Vol.32Issue(1)：48-57,10.DOI:10.11869/j.issn.100-8551.2018.01.0048
竹类植物Dwarf14(D14)基因的多态性分析
Polymorphisms of Dwarf 14 (D14) Gene in Bambusoideae
摘要
Abstract
Strigolactone(SL) is a new plant hormone which regulates shoots branching.DWARF14 (D14) may act as SL receptor,and is also involved in the signal transduction pathway of SL.In order to analyze the correlation between D14 gene and the underground rhizome type,the sequence variations of the gene were investigated in 17 bamboo species that are grouped into scattered,mixed and sympodial bamboos based on their underground rhizome type.All examined orthologous D14 geneswere consisted of two exons and one intron.The DNA,cDNA and amino acid sequences of 17 orthologousD14 genes werefound to have 92.57％,98.97％ and 98.61％ identities,respectively.Their encoded protein sequences contained the catalytic amino acid residues (Ser147,Asp268 and His297) and those amino acid residues (Phe186,Val194,Trp205,Tyr209,Phe245 and Ser270)surrounding the catalyzed substance.The phylogenetic tree based on theD14 gene sequences was generally consistent with the underground rhizome types.The DNA sequences were rich in single nucleotide polymorphism (SNP) sites.The SNP frequency was 1/22,and the nucleotide diversity (π) equaled 0.01476.Seven SNPs were informative in relation to the sympodial bamboos.Real time PCR showed that the expression level of D14 gene was not related to the underground rhizome type.The study can help to recognize the molecular diversity of different underground rhizome types in bamboo and it is the theoretical basis for increasing yields of bamboo shoots.It laid a foundation for further understanding of D14 in the regulation pathway of branching.关键词
竹类植物/独脚金内酯/Dwarf 14(D14)/基因多态性/地下茎类型Key words
bambusoideae/strigolactone/Dwarf 14/gene polymorphism/undergroundrhizome type引用本文复制引用
龚莺,陈虹君,许在恩,郭小勤..竹类植物Dwarf14(D14)基因的多态性分析[J].核农学报,2018,32(1):48-57,10.基金项目
浙江省自然科学基金资助项目(Y307499),浙江省公益项目(2015C32013),国家级大学生创新创业训练计划(201410341004) （Y307499）
