核农学报2018，Vol.32Issue(1)：196-207,12.DOI:10.11869/j.issn.100-8551.2018.01.0196
外源谷胱甘肽对自毒作用下嫁接黄瓜及砧穗幼苗叶片抗氧化系统的影响
Effects of Exogenous Glutathione on Antioxidant System in Leaves of Grafted Cucumber, Stock and Scion Seedlings Under Autotoxicity
摘要
Abstract
The experiment was simulated autotoxicity stress by irrigating soil extract of grafted cucumber with continuous cropping.The leaves were respectively sprayed and rooted by 0,100,200 mg· L-1 GSH,then the biomass,SOD activity,POD activity,APX activity,CAT activity,GSH content,AsA content,MDA content were investigated respectively in the forth day,eighth day,twelfth day,sixteenth day,in order to investigate exogenous GSH effect of relieving autotoxicity and the physiological mechanism about grafted cucumber.The results showed that grafted cucumber,cucumber and pumpkin's seedling index,SOD activity,POD activity,APX activity and CAT activity,GSH and AsA content were restrained,while MDA content was improved by soil extract.So the soil extract of grafted cucumber could produce autotoxicity to three kinds of plant seedlings.T1、T2、T3 of three plants and T4 of grafted cucumber and pumpkin'sound seedling index,SOD activity,POD activity,APX activity and CAT activity,GSH and AsA content were markedly improved,however MDA content was markedly reduced.T4 of cucumber'sound seedling index,SOD activity,POD activity,APX activity and CAT activity,GSH and AsA content were restrained,MDA content was improved.This result showed that applied appropriate exogenous GSH concentration could improve the activities of antioxidant enzymes,the content of antioxidant,and decrease the damage of reactive oxygen species on membrane lipids,thereby effectively promote the growth of grafted cucumber,cucumber and pumpkin seedlings under autotoxicity.The study showed that T4 of grafted cucumber and pumpkin,T2 of cucumber were the better concentraion for alleviating autotoxicity.The experiment provides a theoretical basis for controlling the continuous cropping obstacle of grafted cucumber.关键词
谷胱甘肽/自毒作用/嫁接黄瓜/抗氧化系统Key words
glutathione/auto toxicity/grafted cucumber/antioxidant system引用本文复制引用
于威,颉建明,滕汉玮,胡相莉,裴婕妤..外源谷胱甘肽对自毒作用下嫁接黄瓜及砧穗幼苗叶片抗氧化系统的影响[J].核农学报,2018,32(1):196-207,12.基金项目
甘肃省农业科技创新项目(GNCX-2013-37) （GNCX-2013-37）
