核农学报2018，Vol.32Issue(2)：216-226,11.DOI:10.11869/j.issn.100-8551.2018.02.0216
一个水稻黄绿叶突变体ygl14(t)的鉴定及基因定位
Characterization and Gene Mapping of a Yellow Green Leaf Mutant ygl14 (t) in Rice
摘要
Abstract
Mapping and cloning of genes related to leaf color play an important role in the analysis of the molecular mechanism of leaf color regulation.In this study,ygl14(t) (yellow green leaf mutant 14 temporary) was isolated from the offsprings of an early maturing late japonica variety.Phenotypic analysis showed that ygl14 (t) mutant showed yellow green leaf throughout grown period.Compared with wild type,ygl14(t) had reduced chlorophyll content,aberrant chloroplast development and decreased net photosynthetic rate;and plant height,reduced number of effective panicles,and delayed growth period.Genetic analysis revealed that the yellow green leaf of ygl14(t) mutant was controlled by a single recessive nuclear gene,the mutant gene was mapped in a 40.8 kb region on chromosome 11 between marker D-6 and W1,containing six candidate genes.Sequencing analysis showed that the second base in the junction of the first intron and the second exon mutating from A into T in Os11g0153600,which encodes a chloroplast signal recognition particle 54 kD (cpSRP54) protein,cDNA sequencing analysis confirmed that at the end of the first intron no splicing occurred,there was a 119 bp nucleotides insertion in the coding region,which resulted in the forty-seventh amino acids starting mistranslation.Real-time PCR analysis showed that expression of genes associated with chloroplast development,chlorophyll biosynthesis and photosynthetic system were all up-regulated except rpoC2 was down-regulated and there was unchanged for HEME.The results showed that YGL14(t) affected chloroplast development and is possibly involved in both pigment metabolism and photosynthesis,which lays the foundation for molecular breeding of high photosynthetic efficiency.关键词
水稻/黄绿叶/可变剪接/叶绿体信号识别颗粒Key words
rice/yellow green leaf/alternative splicing/cpSRP引用本文复制引用
林添资,孙立亭,景德道,钱华飞,余波,曾生元,李闯,龚红兵..一个水稻黄绿叶突变体ygl14(t)的鉴定及基因定位[J].核农学报,2018,32(2):216-226,11.基金项目
国家重点研发计划(2017YFD0100400-4),苏省科技支撑计划资助项目(BE2014394),江苏省重点研发计划(现代农业)项目(BE2015363),江苏省“333工程”培养资金资助项目(BRA2014171) （2017YFD0100400-4）
