核农学报2018，Vol.32Issue(2)：227-235,9.DOI:10.11869/j.issn.100-8551.2018.02.0227
不同营养水平水培条件下幼苗期大豆下胚轴性状QTL定位与上位互作分析
Identification of Quantitative Trait Loci and Epistatic Interactions for Seedling Hypocotyl Taits in Soybean Under Different Hydroponic Culture Nutrition Level
摘要
Abstract
In order to study hypocotyl trait under different NPK conditions at seedling stage in soybean,and dig gene resources and reveal the genetic mechanisms.A set of 447 recombinant inbred line (RIL) derived from a cross between Jindou23 (cultivar,female parent) and ZDD2315 (semi-wild,male parent) was used as materials in this study.Epistatic QTL effects and QTLx enviroment interactions were identified in soybean under three treatments:CK (nonfertilized condition),T1 (normal NPK fertilization conditions) and T2 (high fertilization conditions of 1.5NPK).The results showed twenty QTLs for hypocotyl length and weight were detected on 9 chromosomes of 2,6,7,8,9,10,13,16 and 20 using composite interval mapping (CIM) method in this study;the variations accounted for by one signle QTL ranged from 3.65％ to 18.13％.Three and two pair of epistatic QTLs for hypocotyl length and weight was detected respectively non-major QTLs by Compressed mixed linear model (MCIM).The 9 and 10 chromosome contained some QTLs for hypocotyl length and weight in three treatments,and showed that additive effects,additive × environment interactions effects and additive × additive epistatic effects are important genetic factors for hypocotyl length and weight formation and inheritance.The results will give a significant reference for hypocotyl traits QTL mapping,map-based cloning and marker-assisted selection in soybean at the seedling stage,and provide theoretical guidance to fertilization time at seedling stage in soybean and NPK use efficiency in the practical production.关键词
大豆/氮磷钾/下胚轴/QTL/上位性互作Key words
soybean/NPK/hypocotyl/quantitative trait loci/epistatic effects引用本文复制引用
梁慧珍,董薇,许兰杰,余永亮,杨红旗,谭政伟,夏伟,陈鑫伟..不同营养水平水培条件下幼苗期大豆下胚轴性状QTL定位与上位互作分析[J].核农学报,2018,32(2):227-235,9.基金项目
国家现代农业产业技术体系(CARS-21-03B-5),农业科研杰出人才及其创新团队,河南省农业科技攻关计划(152102110140、172102110088) （CARS-21-03B-5）
