核农学报2018，Vol.32Issue(2)：274-282,9.DOI:10.11869/j.issn.100-8551.2018.02.0274
转G10eve基因高抗草甘膦棉花的农艺性状分析
Agronomic Traits of Transgenic Cotton With High Glyphosate Resistance Conferred by Expressing the G10eve Gene
摘要
Abstract
In this study,the G10eve transgenic cotton was used to selected high resistance and stable inheritance lines in the lab and field for cultivating new glyphosate-resistant cotton.The results showed that the resistance level of transgenic cotton line 91 (L91) was evidently higher than line L152 (L152) after spraying different concentration of glyphosate at the cotyledon stage,and the resistance was stable.The accumulation of shikimic acid was obvious different in root,sterm and leaf,and the highest was in leaves.After the treatment of glyphosate,the expression of endogenous EPSPS increased firstly and then decreased.The relative expression level of endogenous EPSPS gene in Coker312 was significantly higher than that in transgenic lines,which further demonstrated that transgenic G10eve gene in cotton could improve the resistance to glyphosate.In the field trials,L91 and AuR (a commercial varieties) could tolerate 20 mL·L-1 glyphosate.When treated with glyphosate at the seedling stage,the plant height,number of branches or number of effective bolls in L91 were increased comparing with the untreated control,while there was no significant difference in those agronomic traits of AuR compared to the control.Moreover,those agronomic traits of L91 and AuR were not inhibited when treated with glyphosate no more than 20 mL· L-1 during the flowering phase.These results implied that L91 could meet the requirements in production.L91 with high resistance level of glyphosate was identified and screened in this work,which provided germplasm resources for cultivating new glyphosate-resistant cotton varieties.关键词
转基因/草甘膦/G10eve/抗性Key words
transgenic/glyphosate/G10eve/resistance引用本文复制引用
陆国清,王春玲,郝宇琼,郭惠明,黄英金,程红梅..转G10eve基因高抗草甘膦棉花的农艺性状分析[J].核农学报,2018,32(2):274-282,9.基金项目
国家转基因重大专项(2016ZX08005-004),中国农业科学院科技创新工程 （2016ZX08005-004）
