核农学报2018，Vol.32Issue(2)：389-398,10.DOI:10.11869/j.issn.100-8551.2018.02.0389
环境中纳米TiO2对拟南芥生长及相关基因表达的影响
Effects of Nano-TiO2 on Growth and Gene Expression in Arabidopsis thaliana
摘要
Abstract
To investigate the mechanism of nano-TiO2 toxicity in environment,this research studied the effects of different concentrations (0.05,0.1,0.5,1,1.5 mg·L-1) nano-TiO2 on seed germination,growth and gene transcription levels of Arabidopsis thaliana.The results showed that compared with the control group,1.00 mg· L-1 and 1.50 mg· L-1 treated-groups:the seed germination rate,germination potential and germination index were inhibited;The plant height,leaf area and chlorophyll content of seedlings decreased,and root length and root vigor went up.In plant root,the IAA content showed an upward trend while the ABA content was decreased.Under the treatment of 1.50 mg· L-1,the contents of IAA and ABA in leaves decreased and increased,respectively.Lhcb3,CAO,Rubisco and RCA gene expression were first increased and then decreased.The results indicated that nano-TiO2 slowed down the seed germination,inhibited the photosystem Ⅱ activity,carbon assimilation and photosynthetic carbon metabolism,decreased photosynthesis efficiency and finally restrained the growth of Arabidopsis thaliana.The results provide a theoretical basis for further investigating the molecular mechanism of the toxic effects of nano-TiO2 on plant toxicity and its biological effects in the ecological environment,and also provid provide the basis for environmental safety evaluation of nanomaterials.关键词
纳米TiO2/拟南芥/生长/基因表达Key words
nano-TiO2/Arabidopsis thaliana/growth/gene expression引用本文复制引用
兰丽贞,赵群芬,金凯星..环境中纳米TiO2对拟南芥生长及相关基因表达的影响[J].核农学报,2018,32(2):389-398,10.基金项目
浙江省自然科学基金(ZX2013000355) （ZX2013000355）
