核农学报2018，Vol.32Issue(4)：646-653,8.DOI:10.11869/j.issn.100-8551.2018.04.0646
青花菜转录因子基因BoNAC1的克隆与表达分析
Isolation and Expression Analysis of Broccoli Transcription Factor Gene BoNAC1
摘要
Abstract
To investigate sequence characterristics and expression features of the Brassica oleracea var.italica NAC transcription factor gene,in this study,broccoli was used as the experimental materials a NAC transcription factor gene designated BoNAC1 was isolated Brassica oleracea var.italica by using PCR method,and analyzed by Real-time quantitative PCR to clarify its expression patterns inoculationd by Sclerotinia sclerotiorum and Plasmodiophora brassicae.The results indicated that the BoNAC1 contained two introns and their lengthis 1 832 bp and 670 bp respectively,and encoded 344 amino acids with a NAM domain.The results of phylogenetic tree indicated BoNACl has a close relationship with NACs from other Brassica plants,and they grouped with NACs from other Cruciferae plants,however,a far relationship was observed between BoNAC1 and NACs from Leguminosae and Rosaceae plants,and they clustered in different clades.Expression analysis revealed BoNAC1 was induced by Sclerotinia sclerotiorum,and the highest expression levels were detected after 12 h and 24 h incubation,and the fold changes were 6.26 and 7.03 as high as that of the control;Furthermore,the expression of BoNAC1 also induced by Plasmodiophora brassicae,and the highest expression levels were observed after 15 d and 20 d incubation,and the fold changes were 4.23 and 4.11 as compared to the control,indicting that this gene was involved in response to both Sclerotinia sclerotiorum and Plasmodiophora brassicae interactions.Cloning and expression analysis of BoNAC1 in this study,which can provide the theoretical basis for understanding the gene function idenfication and its application in the future.关键词
NAC/青花菜/表达/核盘菌/根肿菌Key words
NAC/broccoli/expression/Sclerotinia sclerotiorum/Plasmodiophora brassicae引用本文复制引用
蒋明,张慧娟,张志仙,陈珍,管铭,刘洁,陈孝赏..青花菜转录因子基因BoNAC1的克隆与表达分析[J].核农学报,2018,32(4):646-653,8.基金项目
台州市科技计划项目(162ny14),浙江省公益性技术应用研究计划项目(2016C32091) （162ny14）
