华西口腔医学杂志2018，Vol.36Issue(1)：17-22,6.DOI:10.7518/hxkq.2018.01.004
丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价
Preparation and characterization of oriented scaffolds derived from cartilage extracellular matrix and silk fibroin
摘要
Abstract
Objective This study aims to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and silk fibroin (SF) and use to investigate their physicochemical property in cartilage tissue engineering.Methods Oriented SF-CECM scaffolds were prepared from 6％ mixed slurry (CECM:SF=1 ∶ 1) through modified temperature gradient-guided thermal-induced phase separation,followed by freeze drying.The SF-CECM scaffolds were evaluated by scanning electron microscopy (SEM) and histological staining analyses and determination of porosity,water absorption,and compressive elastic modulus of the materials.Results The SEM image showed that the SF-CECM scaffolds contained homogeneous reticular porous structures in the cross-section and vertical tubular structures in the longitudinal sections.Histological staining showed that cells were completely removed,and the hybrid scaffolds retained proteoglycan and collagen.The composition of the scaffold was similar to that of natural cartilage.The porosity,water absorption rate,and vertical compressive elastic modulus of the scaffolds were 95.733％±1.010％,94.309％±1.302％,and (65.40±4.09) kPa,respectively.Conclusion The fabricated SF-CECM scaffolds exhibit satisfactory physicochemical and biomechanical properties and thus could be an ideal scaffold in cartilage tissue engineering.关键词
软骨/丝素蛋白/细胞外基质/取向支架/组织工程Key words
cartilage/silk fibroin/extracellular matrix/oriented scaffolds/tissue engineering分类
生物科学引用本文复制引用
滕彬宏,赵艳红,王连永,杨强,李洪发,李云洁..丝素蛋白-软骨脱细胞外基质复合取向支架的制备及评价[J].华西口腔医学杂志,2018,36(1):17-22,6.基金项目
国家自然科学基金(31300798,31470937,81572154) （31300798,31470937,81572154）
天津市卫生局科技攻关重点项目(15KG125,16KG114)The National Natural Science Foundation of China (31300798,31470937,81572154) （15KG125,16KG114）
The Key Project of Science and Technology Research of Tianjin Health Bureau (15KG125,16KG114). （15KG125,16KG114）
