华西口腔医学杂志2018，Vol.36Issue(1)：76-81,6.DOI:10.7518/hxkq.2018.01.015
牙龈卟啉单胞菌精氨酸特异性牙龈素基因疫苗预防比格犬种植体周围炎的实验研究
Experimental research on Arginine-gingipain A gene vaccine from Porphyromonas gingivalis that prevents peri-implantitis in Beagle dogs
摘要
Abstract
Objective This study aims to use Arginine-gingipain A gene vaccine (pVAX1-rgpA) to immunize adult Beagle dogs and to evaluate its effect during peri-implantitis progression and development.Methods Plasmid pVAX1-rgpA was constructed.The second and third bilateral mandible premolars of 15 adult Beagle dogs were extracted,and the implants were placed immediately.After 3 months,the animals were randomly divided into groups A,B,and C.Afterward,the animals were immunized thrice with plasmid pVAX1-rgpA,with heat-killed Porphyromonas gingivalis,or pVAX1,respectively.IgG in the serum and secretory IgA (sIgA) in saliva were quantitatively analyzed by enzyme-linked immunosorbent assay before and after 2 weeks of immunization.Peri-implantitis was induced with cotton ligatures fixed around the neck of implants.Probing depth (PD) and bleeding on probing were recorded.All animals were sacrificed after ligaturation for 6 weeks.Decalcified sections with thickness of 50 μm were prepared and dyed with methylene blue to observe the bone phenotype around implants.Results Levels of serum IgG and sIgA in saliva were higher in groups A and B after immunization than before the process (P＜0.05) and higher than those in group C (P＜0.05).However,no difference was observed between groups A and B (P＞0.05).At 4 and 6 weeks after ligaturation,PD of the ligatured side in group C was higher than that in groups A and B (P＜0.05).On the other hand,no difference was identified between groups A and B (P＞0.05).Bone loss in group A was significantly lower than that of the other groups (P＜0.05).Abundant inflammatory cells and bacteria were present in the bone loss area around the implants in the three groups,as identified through hard tissue section observation.However,group C presented the most number of inflammatory cells and bacteria in the bone loss area around the implants.Conclusion IgG and sIgA can be generated by immunity with rgpA DNA vaccine,which can significantly slow down bone loss during experimental peri-implantitis in dogs.关键词
基因疫苗/牙种植体/种植体周围炎/精氨酸特异性牙龈素/牙龈卟啉单胞菌Key words
gene vaccine/dental implant/peri-implantitis/Arginine-gingipains/Porphyromonas gingivalis分类
医药卫生引用本文复制引用
李传花,王志峰,朱丽娜,范欣,蓝菁..牙龈卟啉单胞菌精氨酸特异性牙龈素基因疫苗预防比格犬种植体周围炎的实验研究[J].华西口腔医学杂志,2018,36(1):76-81,6.基金项目
国家自然科学基金(30801308) （30801308）
山东省科技厅攻关项目(2010G0020237)The National Natural Science Foundation of China (30801308) （2010G0020237）
Foundation of Department of Science and Technology of Shandong Province (2010G0020237). （2010G0020237）
