华中师范大学学报（自然科学版）2018，Vol.52Issue(1)：53-57,5.DOI:10.19603/j.cnki.1000-1190.2018.01.010
蛇菰内生菌Aspergillus sp.SHG-7代谢产物研究
Study on secondary metabolites from endophytic fungus Aspergillus sp.SHG-7 isolated from Balanophora henryi Hesml
摘要
Abstract
In the present work,the fungus SHG-7 strains was isolated from Balanophora henryi Hesml.and classified as Aspergillus sp.based on morphological features and its sequence.Six compounds were generated from the its fermentation broth by thin layer chromatography,Sephadex LH-20 gel column chromatography and preparative HPLC,which were elucidated as griseofulvin (1),ergosterol(2),ergosta-7,22-dien-3,6-dione (3),ergosta-7,22-diene-3,5,6-triol (4),glycerol-1-hexadecanoate (5),and myristic acid(6) by means of MS and NMR.The results of biological activity assay showed that compound 1 had strong cytotoxic activities against MCF 7,A549 and Hela cell with IC50 18.36 ± 2.32 μM,26.58 ± 1.62 μM and 74.29 ± 1.34 μM,respectively.Compound 2-6 had no significant activity to these tumor cells.关键词
蛇菰/内生真菌/次级代谢产物/细胞毒活性Key words
Balanophora henryi Hesml/endophytic fungi/secondary metabolites/cytotoxic activity分类
医药卫生引用本文复制引用
张宏岐,柳蔚,高星星,郑倩倩,罗菲..蛇菰内生菌Aspergillus sp.SHG-7代谢产物研究[J].华中师范大学学报（自然科学版）,2018,52(1):53-57,5.基金项目
湖北省教育厅科研项目(Q20161204). （Q20161204）
