吉林农业大学学报2018，Vol.40Issue(1)：34-42,9.DOI:10.13327/j.jjlau.2017.3475
防风枯斑病的病原学及药剂敏感性
Pathogen and Fungicide Susceptibility of Saposhnikovia divaricata Blight Caused by Diaporthe angelicae
摘要
Abstract
Biological characteristics of Diaporthe angelicae (a new plant disease in China) and its susceptibility to fungicides were studied.Single spore isolation method was used to obtain 3 isolates.The pathogen which caused Saposhnikovia divaricata blight were identified as Diaporthe angelicae based on morphological characteristics,cultural characteristics and rDNA-ITS,TUB2 and TEF sequence analysis.The results of the biological characteristics of the pathogen showed that the suitable temperature for mycelial growth and spore production was 25 ℃,and the suitable pH was 8;Maltose and Sucrose as the main carbon sources were optimal for mycelial growth and spore production,Asparagine was the main nitrogen sources for mycelial growth and spore production.The germination rate of the conidia was the highest at 25 ℃,and the lethal temperature of the conidia was 35 ℃.The germination rate of the conidia was the highest when pH was 8.Light had positive effect on conidia germination.The mycelial growth rate was used to test the susceptibility of the pathogen to 23 fungicides.The pathogen was more sensitive to 30％ triflumizole WP,3％ hexaconazole,27％ isoprothiolane EC,40％ flusilazole EC,55％ flusilazole · carbendazim WP,25％ propiconazol EC,30％ difenoconazole SC,30％ kresoxim-methyl WP,250 mg/L pyraclostrobin EC,10％ difenoconazole WG,and 77％ copper calcium sulphate WP,the EC50 of which was all less than 0.1 mg/L.关键词
防风/枯斑病/病原鉴定/药剂敏感性Key words
Saposhnikovia divaricata/blight/pathogen identification/fungicide susceptibility分类
农业科技引用本文复制引用
王梦奇,刘丽玲,李海洋,魏春艳,白庆荣..防风枯斑病的病原学及药剂敏感性[J].吉林农业大学学报,2018,40(1):34-42,9.基金项目
国家自然科学基金项目(31500022),国家质检总局科技计划项目(2013IK276),吉林省教育厅项目[吉教合字(2014)第463号] （31500022）
