吉林农业大学学报2018，Vol.40Issue(1)：71-79,9.DOI:10.13327/j.jjlau.2017.3611
玉米根际溶磷菌Bucillus megaterium×S2的筛选鉴定与溶磷特性分析
Screening, Identification and Phosphate-solubilizing Property Analysis of Inorganic Phosphate-solubilizing Bacteria Bucillus megaterium XS2 Isolated from Maize Rhizosphere Soil
摘要
Abstract
In order to fully mobilize phosphorus resources of soil,reduce the application amount of chemical phosphorus fertilizer,and then reduce agricultural non-point source pollution caused by the application of phosphate fertilizer,one phosphate-solubilizing bacterium named XS2 was isolated from rhizosphere soil of maize by PVK medium and identified by colony morphology characteristics,physiological and biochemical tests and 16S rDNA sequence analysis.The phosphate-solubilizing conditions were optimized in 3 aspects of carbon source,nitrogen source and pH value by using L25(53) orthogonal tests,the dissolution characteristics of strain XS2 were determined for a variety of insoluble phosphate sources,the changes of organic acids in the fermentation broth of strain XS2 were analyzed by HPLC and the phosphate-dissolving mechanism was primarily analyzed.The results show that strain XS2 belonged to Bucillus megaterium,and the phosphate-solubilizing capacity reached the highest level of 401.33 mg/L under the conditions of glucose as carbon source,ammonium sulfate as nitrogen source and pH 5.The strain had better ability to dissolve Ca3(PO4)2,A13(PO4)2,FePO4 and phosphate powder.Oxalic acid,malic acid,citric acid,lactic acid,acetic acid,acetic anhydride and several unknown acids were determined by HPLC and the kinds and contents of organic acids in culture medium were different with the change of culture time.Citric acid,lactic acid,acetic acid and oxalic acid seemed to be the major organic acids which affect the dissolution of phosphorus.A strain of Bacillus megaterium XS2 was obtained and identified as P.decumbens.It can convert insoluble phosphorus into available phosphorus for plants,increasing content of effective phosphorus in soil and crop yield and providing data and theoretical basis for the solution of widespread pollution caused by the overuse of phosphate fertilizer from the source.关键词
农业面源污染/溶磷菌/溶磷特性/有机酸Key words
agricultural non-point source pollution/phosphate-solubilizing bacteria/phosphate-solubilizing property/organic acid分类
农业科技引用本文复制引用
萧龙珍,崔俊涛,刘淑霞,窦森,王继红,王呈玉..玉米根际溶磷菌Bucillus megaterium×S2的筛选鉴定与溶磷特性分析[J].吉林农业大学学报,2018,40(1):71-79,9.基金项目
地方高校国家级大学生创新创业训练计划项目(201410193010),吉林省科技发展计划项目(20140204043NY),国家自然科学基金项目(31301850) （201410193010）
