吉林农业大学学报2018,Vol.40Issue(1):80-84,5.DOI:10.13327/j.jjlau.2017.3797
中华散尾鬼笔提取工艺响应面优化及其粗提物毒力测定
Optimization of Extracts from Lysurus mokusin Using Response Surface Methodology and Their Toxicity Test
摘要
Abstract
Lysurus mokusin crude extract (LMCE) from Lysurus mokusin by ultrasonic method was used as an index in this test.The effects of different ultrasonic time,ethanol concentration,ultrasonic power and solid-liquid ratio on extracting rate of LMCE were investigated by single factor experiment,then the three factors,ultrasonic time,ultrasonic power and solid-liquid ratio,were chosen to determine the optimum process parameters in the response surface test.The results showed that under the conditions of 80 min of ultrasonic time,60% ethanol and 1 ∶ 80(g ∶ mL) of solid-liquid ratio,the extraction rate of LMCE was the highest (31.21%).The effects of LMCE on gypsy moth larvae were studied.The results showed that the adjusted mortality for the larvae of gypsy moth larvae was 59.77%.关键词
中华散尾鬼笔/舞毒蛾/响应面优化/提取工艺/毒力测定Key words
Lysurus mokusin/gypsy moth/optimization of response surface/extraction process/toxicity test分类
化学化工引用本文复制引用
林连男,王瑞琦,张国珍,王亚军,张晓波,张国财..中华散尾鬼笔提取工艺响应面优化及其粗提物毒力测定[J].吉林农业大学学报,2018,40(1):80-84,5.基金项目
中央高校基本科研业务费专项资金项目(2572016AA03),哈尔滨市应用技术研究与开发项目(2016RAXXJ035),黑龙江省森工总局科研项目(sgzjT2015005) (2572016AA03)