寄生虫与医学昆虫学报2017，Vol.24Issue(3)：141-147,7.DOI:10.3969/j.issn.1005-0507.2017.03.001
新疆地区扇头蜱中Candidatus Rickettsia barbariae的检测与序列分析
DETECTION AND SEQUENCES ANALYSIS OF CANDIDATUS RICKETTSIA BARBARIAE IN RHIPICEPHALUS TICKS FROM XINJIANG
摘要
Abstract
To investigate the prevalence of Candidatus Rickettsia barbariae in ticks from Xinjiang,total of 90 Rhipicephalus sanguineus and 420 Rhipicephalus turanicus were collected from Yuli area and the genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detect with PCR.The target genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detected from 10 Rh.sanguineus and 53 Rh.turanicus with rates as 11.11％ and 12.62％ respectively.The sequences obtained from these ticks were identical to the corresponding sequence in the positive samples.By BLAST analysis,the ompA,ompB partial sequences were 99.8％ (583/584) and 100％ (768/768) identical to the corresponding sequence of Candidatus Rickettsia barbariae strain from Rh.turanicus in Italy (EU272186.1,EU272187.1) respectively.The gltA and 17kDa partial sequence were also 100％ identical (375/375,356/356) to the corresponding sequence of Candidatus Rickettsia barbariae strain from Vermipsylla alakurt in Xinjiang (KT284716.1,KT284715.1).The manifestations of Candidatus Rickettsia barbariae occur in Rh.sanguineus and Rh.turanicus might suggest the potential health threat of the rickettsia organism to residents in Xinjiang area.关键词
血红扇头蜱/图兰扇头蜱/Candidatus Rickettsia barbariae/斑点热群立克次体/新疆Key words
Rhipicephalus sanguineus/Rhipicephalus turanicus/Candidatus Rickettsia barbariae/Spotted fever group rickettsia/Xinjiang引用本文复制引用
孙响,张桂林,郑重,刘然,邱尔臣..新疆地区扇头蜱中Candidatus Rickettsia barbariae的检测与序列分析[J].寄生虫与医学昆虫学报,2017,24(3):141-147,7.基金项目
国家自然科学基金项目(U1303104) （U1303104）
