江西农业学报2018，Vol.30Issue(3)：112-115,121,5.DOI:10.19386/j.cnki.jxnyxb.2018.03.23
HPLC法同时测定4种焙炒咖啡豆中6种有机酸方法初探
A Primary Research on Simultaneous Determination of Six Organic Acids in Four Kinds of Roasted Coffee Beans by HPLC
摘要
Abstract
A HPLC method for the simultaneous determination of citric acid,malic acid,tartaric acid,succinic acid,gallic acid and fumaric acid in roasted coffee beans was constructed,and the contents of these organic acids in 4 kinds of roasted coffee beans were detected and compared by using this method. The extraction conditions for samples were confirmed from the aspects of grinding fineness, dosing weight, water temperature and brewing time. The HPLC conditions were ascertained as follows: Ac-claimTM120 C18chromatographic column(4.6 mm×250 nm,5 μm);mobile phase methyl alcohol plus 0.1% phosphoric acid(25:75); flow rate 1 mL/min; column temperature 30 ℃; determination wavelength 210 nm. The results indicated that: under the conditions of extracting 5 g ground coffee from roasted coffee beans by using normal-temperature pure water,using 3-grade coffee grinder to grind the ground coffee, and conducting ultrasonic dissolving for 5 min in 40℃ water,citric acid,malic acid,tartaric acid,succinic acid,gallic acid and fumaric acid could be extracted from roasted coffee beans very well. The linear correlation co-efficients(R2)of tartaric acid, malic acid, citric acid, succinic acid, gallic acid and fumaric acid were 0.9992, 0.9997, 1. 0000,0.9999,1.0000 and 1.0000 respectively within the range of 2.5~30,1.25~15.00,2~24,0.5~6.0,0.025~0.300 and 0.025~0.300 μg, and their standard-adding recoveries were 98.01%, 100.29%, 110.12%, 96.55%, 98.3% and 106.32%separately. Thus, this method was simple, convenient and quick with good reproducibility in use, and it could be used to detect the organic acids in coffee beans.关键词
HPLC/柠檬酸/苹果酸/酒石酸/琥珀酸/没食子酸/富马酸/焙炒咖啡豆Key words
HPLC/Citric acid/Malic acid/Tartaric acid/Succinic acid/Gallic acid/Fumaric acid/Roasted coffee bean分类
农业科技引用本文复制引用
武瑞瑞,李贵平,黄健,李亚男,杨阳,黄家雄..HPLC法同时测定4种焙炒咖啡豆中6种有机酸方法初探[J].江西农业学报,2018,30(3):112-115,121,5.基金项目
横向项目"潞江坝不同海拔高度小粒种咖啡农艺观测与品质分析" （）
云南省农科院科技专项"精品咖啡新品种选育及新技术集成示范应用"(2017RA01414). （2017RA01414）
