检验医学与临床2017，Vol.14Issue(23)：3478-3480,3.DOI:10.3969/j.issn.1672-9455.2017.23.019
结核合并感染凝固酶阴性葡萄球菌患者的耐药性分析及耐药基因检测
Analysis of drug resistance and drug resistance gene detection of TB co-infected with coagulase negative Staphylococcus
摘要
Abstract
Objective To investigate the distribution of drug resistance gene in patients with tuberculosis(TB) co-infected with coagulase negative Staphylococcus(CNS),so as to provide guidance to fight TB co-infections.Methods Patients in our hospital who confirmed tuberculosis complicated with infections were collected,and drug resistance of 54 strains of CNS were analyzed,and polymerase chain reaction(PCR) was used to detect the resistance genes.Results Drug susceptibility of 54 strains of CNS showed that,the resistance rate to benzene azole Westwood coagulase negative Staphylococcus(MRCNS) detection was 92.3 ％,the resistant rate of penicillin was 96.2 ％,which of erythromycin,gentamicin and tetracycline were 86.5 ％,75.0 ％ and 86.5 ％,respectively.Strain which resistant to vancomycin intermediary was also found.PCR amplification showed that the 50 strains had mecA gene,46 strains had aac(6')/aph(2') gene,26 strains had aph(3')Ⅲ gene,20 strains had ermA gene,15 strains had tetM gene,and positive rates were 92.5 ％,85.1％,48.1％,37.0 ％ and 27.8 ％,respectively.Conclusion TB associated infections of CNS has showed multidrug resistance,carrying a variety of resistance genes,and drug resistance is serious,resistance genes and resistance are closely re lated.关键词
结核/凝固酶阴性葡萄球菌/耐药基因/耐药性Key words
tuberculosis/coagulase negative Staphylococcus/resistance genes/drug resistance引用本文复制引用
王锦萍,蔡常辉,梁连辉,陈述文,杨海辉..结核合并感染凝固酶阴性葡萄球菌患者的耐药性分析及耐药基因检测[J].检验医学与临床,2017,14(23):3478-3480,3.基金项目
广东省中山市医学科研项目(2015A020226). （2015A020226）
